Detailed Information [140056]
 

Strain Information
DGRC Number 140056
Genotype with FlyBase Link y[*] w[*]; PBac{SAstopDsRed}LL00259 P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM3, Sb[1]
Genotype y* w*; PBac{SAstopDsRed}LL00259 P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E / TM3, Sb1
Break points/Insertion Site 62D5
Related Genes CR32303
Received Date 19 October 2007
Original Number LL00259
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3L:2273667(+)
Cytological Band: 62D5
Gene Symbol-1: CR32303
CG Number-1: CR32303
FlyBase ID-1: FBgn0052303
Insertion Type-1: Putative Promoter
Gene Symbol-2:
CG Number-2:
FlyBase ID-2:
Insertion Type-2:
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Lethality semi lethal
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]
Stock Request

Library & Clone Information
Strand Plus
Insertion Point 2273667
Chromosome Band 3L
Flanking Sequence 
NNNNNNNNGCATGCGTGATTTNACCCTGACTATCTTTCTAGGATTAACATGCTGCATTGG
CGAACTTTTATCCCATCTGGGAACTTTAAAAGGCGCTCTGAGTTCGCCATTTTCAAGTCG
GGGGTATAGCTCAGGGGTAGAGCATTCGAATTAACCATTGTGGGAACACTAGAACTAGCT
AGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAATTCGCCCTATAGTGAGTCGTATTA
CGTTATCTAGTTAGGCGCGCCTGTGGGACGGAAGAACAGATGAATTAGATATCTATAACN
AGAAAATATATATATTATTANTTATCNCGTAAGTAGAACCTGAANTAACATATAATTANC
CNATGGAGTTAAACTTAAAAGTCCCGTAAAAGANTANCCTGCNTCAAANCCAAAAANGAG
GTTNTTNNNTTNCNNTTTAAGNNCNTNNNNGGNNNNNNNANNNNTGAATTANNNNNTNNN
NNGAAATANANNNTNNANNTATCNNNNN
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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