Detailed Information [140223]
 

Strain Information
DGRC Number 140223
Genotype with FlyBase Link y[*] w[*]; PBac{SAstopDsRed}LL01005 P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; PBac{SAstopDsRed}LL01005 P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 65E4
Related Genes form3 (CG33556)
Received Date 19 October 2007
Original Number LL01005
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3L:7100376(-)
Cytological Band: 65E4
Gene Symbol-1: form3
CG Number-1: CG33556
FlyBase ID-1: FBgn0053556
Insertion Type-1: Intron
Gene Symbol-2:
CG Number-2:
FlyBase ID-2:
Insertion Type-2:
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
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Library & Clone Information
Strand Minus
Insertion Point 7100376
Chromosome Band 3L
Flanking Sequence
tttacgcagactatctttctagggttaaCGATAACAACATAAGTAATATTGCYGCCTTGT
AATAATATTATTATTTACTTATGTAACAACTGGATATTTGTAAAGTATGTCTGCACTCTT
GATTTATGTTAATTGAGATCTATGCTTATTTAAAGAATATGAGTACCTAAATTGTTAAAT
ATTTTTTTTTTTTTTKWAWYYMAAAARGGYCCARGGRAAWKGTTTTTTTTYTTTWAATTT
TTTTTTTTTTCAAATWACCCTTTGGGGGAACMCWAAAACWRSYTRKTTYWRGGGGGSCCC
CCCCCSSGGGGGASCCCCAWTTCCCCCTWWRGGGRGYSKWWTWMCKTTWYCWRKTKGGGC
GCGCCGTKGGRMSGRAARAMCARWTRAWTTAKATTTCTWTAMCARRAAAWTWTWTWTWTA
ATAGKTTMTCRCGTARGTRGAACATGAAWTAMCAWTWTATTTWTSGTRKGAKTWAAWCYT
WAAARKYCMCGWAAAARWTWMTCKYGCSYAMCGAASTWTATTCATGCKTACGTTAAAWTT
TMWTATWTTTCCTAAMMCCGCGCGCCGCGCGYGMKATGTTGTGTWGTKATAAATAAAAAA
ASAKAATAATKAGWTTYTTRTTTTYCTAT
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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