Detailed Information [140374]
 

Strain Information
DGRC Number 140374
Genotype with FlyBase Link y[*] w[*]; PBac{SAstopDsRed}LL01471 P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; PBac{SAstopDsRed}LL01471 P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 75B2
Related Genes Eip75B (CG8127)
Received Date 19 October 2007
Original Number LL01471
Chromosome 3
Comments No Tb expression but Hu is OK. 22Dec2021 E.O.
Original Source Liqun Luo, Stanford University
Original Comments
Location: ~3L:17983551(-)
Cytological Band: "75B2"
Gene Symbol-1: Eip75B
CG Number-1: CG8127
FlyBase ID-1: FBgn0000568
Insertion Type-1: Intron
Gene Symbol-2:
CG Number-2:
FlyBase ID-2:
Insertion Type-2:
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Lethality lethal
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2021-12-22
Research papers using this strain
[Please submit your publication]
Stock Request

Library & Clone Information
Strand Minus
Insertion Point 17983551
Chromosome Band 3L
Flanking Sequence 
GMTTCAGATGCATGCGTCATTTACGGAGACAGATGAATTAGATATCTATAACAAGAAAAT
ATATATATAAGYCTGCTWAMAMRWAAGTAGAACATGAAATAACAATATAATTATCKTWTK
RGKWAAATCTTAAAAGTCACGTAAAAGATAATCATGCGTCAYKTTGGCTCTYAMTTTTCA
WTAARWTGCCGGGGGGCTTTTCKTGTTGSWCAGCTTTCTTTATTTCTGATTCTGGGCCTA
TWATCTAACTKGTTTGTTATTCCTTCCACATTATTTAGCATATTTATTTATTTACCCACA
CTCRGGCATACAATTTTGTATTTTCTTTCTTTTTTTTTTTKKSSSGCGSSKGKKGGGRRG
SCTCKSKTTTWATTTTTYGKTTCCCTTTTCSCTCGGSMGGTGTTGGGKWAKCTTCCGTGT
RTTTTTCGCGCYTGGGAAGGGGAGGGGGGCKRTTWTTTTTGKAGGGGGCSCTCCRTTTGT
GKGCGKCCGCMAAATGTRAAKAACCKCSTCCAWGGCAAASCAGAAACRAAASCSGSTTAC
GCMGCAWGGSCMACRARAAACCAKGGAGGGAGGSGSGGARAAAGAAGARACCTTMCCCYC
YCCCGCTCTTTGGWWTGGCTTTTGGGTTTAGGGTTTCCCGSSCAYTCCAAWRKTTTTTGT
GTYCKTTTAAGCWTTCCAAARRSMYCTTCCCCCCAMCTTTYTTTMAAAYKWTCCSTTTTT
TTYAATTCTMCAASACCYMCCCGRCCCWGTATTAGWCTCTCCCARKATTAWTTKGTTAYR
MACAAACSACAATACGCCYTTGAA
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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