Detailed Information [109042]

Strain Information
DGRC Number 109042
Genotype with FlyBase Link w[1118]; P{BmDelta-w}ken[ok] P{ken[+t9]}1-6 / SM1
Genotype w1118; P{BmΔ-w}kenok P{ken+t9}1-6 / SM1
Original Number kN07-D4
Comments No or little Cy expression. 14May2014 M.W.
Original Source Daisuke Yamamoto, Waseda Univeristy
Original Comments line#1-6. The genomic ken[+] transgene was constructed by inserting the genomic sequence between nt. 8959(HindIII) and nt. 18,011(PstI) into the pCasper3 vector (the nucleotide numbers were defined in the genomic map described in Lukacsovich et al., 1999, Genomics 57, 43-56). The genomic ken[+] completely rescued the ken mutant phenotypes, unlike the hs-ken[+] and UAS-ken[+] transgenes. The gemomic region surrounding the ken locus is extremely dence in genes; 10 genes have been identified molecularly within the 45 kB stretch. Some of them physically overlap with each other. Thus the genomic ken[+] line would be useful to distinguish these adjacent transcription units by means of genetic complementation tests. The genomic ken[+] transgene inserted intoX (DGRC# 109043, original# kN14-C1), 2nd (DGRC# 109042, original# kN07-D4) and third (DGRC# 109044, original# kN14-D1) chromosomes was equally effective in rescuing the ken mutant phenotypes. The stock with the 2nd chromosome insertion of the genomic ken[+] transgene also carries the P{BmDelta-w}ken[ok] mutation on the same chromosome.
Genus Drosophila
Subgenus Sophophora
Species Group melanooaster
Species Subgroup melanogaster
Species melanogaster
Reference Lukacsovich T, Yuge K, Awano W, Asztalos Z, Kondo S, Juni N, Yamamoto D.
The ken and barbie gene encoding a putative transcription factor with a BTB domain and three zinc finger motifs functions in terminalia development of Drosophila.
Arch. Insect Biochem. Physiol. (2003) 54(2) 77-94 [RRC reference]
Last update 2017-11-23
Research papers using this strain
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Lukacsovich T, Yuge K, Awano W, Asztalos Z, Kondo S, Juni N, Yamamoto D.
The ken and barbie gene encoding a putative transcription factor with a BTB domain and three zinc finger motifs functions in terminalia development of Drosophila.
Arch. Insect Biochem. Physiol. (2003) 54(2) 77-94 [PubMed ID = 14518006] [RRC reference]
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