Detailed Information [109611]

Strain Information
DGRC Number 109611
Genotype with FlyBase Link sc[*] z[1] w[+(TE)] mei-9[a] mei-41[D5] / FM7c; mwh[*]
Genotype sc* z1 w+(TE) mei-9a mei-41D5 / FM7c; mwh*
Received Date 4 June 2009
Chromosome 1
Comments Bristles are one-half to two-thirds normal length. 6Feb2013 T.O.
See Drosophila Mutagenicity Test
Original Source Hiroshi Baba, Mitsubishi Tanabe Pharma Corporation
Original Comments This strain was established by Inoue of Mitsubishi Chemical Corporation as a tester strain for simultaneous screening of genotoxic, mutagenic and clastogenic activity of chemical compounds. This assay, referred to as a combined assay for the DNA repair test and the wing spot test (somatic mutation and recombination test), is suited for screening mutagenic/carcinogenic activity of chemical compounds 1), 2), 3).

The strain carries two types of X chromosome. One is the DNA repair-defective X-chromosome (sc z[1] w[+(TE)] mei-9[a] mei-41[D5]) and the other is the X chromosome balancer ( In(1)FM7 y[31D] sc[8] dm B / (hereafter designated as FM7 / mei-9 mei-41). The X chromosomal genes, mei-9[a] and mei-41[D5], are an excision-repair defective and a post-replication repair defective mutations, respectively. The third chromosome carries multiple wing hair (mwh[*]), a wing-cuticle marker which confers multiple wing hairs in a single cell. With regard to DNA repair capability, the stock consists of the following four types of flies:

  • mei-9 mei-41 / mei-9 mei-41 females (DNA repair defective)
  • FM7 / mei-9 mei-41 females (DNA repair proficient)
  • mei-9 mei-41 / Y males (DNA repair defective)
  • FM7 / Y males (DNA repair proficient)
In the combined assay, FM7 / mei-9 mei-41; mwh / mwh females are mated with wild-type males (mei-9+ mei-41+; mwh+ / mwh+) and the F1 larvae are treated with a test compound. The insecticide-resistant Oregon R(R) strain is mostly used in place of the wild-type males to increase the test sensitivity for screening mutagens/carcinogens which requires metabolic activation.

<References>
1) Inoue H., H. Baba, K. Awano and K. Yoshikawa (1995): Genotoxic effect of griseofulvin in somatic cells of Drosophila melanogaster, Mutation Research 343, 229-234 doi:10.1016/0165-1218(95)90018-7
2) Graf, U., F.E. Würgler, A.J. Katz, H. Frei, H. Juon, C.B. Hall and P.G. Kale (1984): Somatic mutation and recombination test in Drosophila melanogaster, Environmental Mutagenesis, 6, 153-188 doi: 10.1002/em.2860060206
3) Fujikawa, K. F. L. Fort, K. Samejima and Y. Sakamoto (1993): Genotoxic potency in Drosophila melanogaster of selected aromatic amines and polycyclic aromatic hydrocarbons as assayed in the DNA repair test, Mutation Research, 290, 175-182 doi:10.1016/0027-5107(93)90157-B

Genus Drosophila
Subgenus Sophophora
Species Group melanooaster
Species Subgroup melanogaster
Species melanogaster
Reference Inoue H, Baba H, Awano K, Yoshikawa K.
Genotoxic effect of griseofulvin in somatic cells of Drosophila melanogaster.
Mutat. Res. (1995) 343(4) 229-34 [RRC reference]

Graf U, Wrgler FE, Katz AJ, Frei H, Juon H, Hall CB, Kale PG.
Somatic mutation and recombination test in Drosophila melanogaster.
Environ Mutagen (1984) 6(2) 153-88 [RRC reference]

Fujikawa K, Fort FL, Samejima K, Sakamoto Y.
Genotoxic potency in Drosophila melanogaster of selected aromatic amines and polycyclic aromatic hydrocarbons as assayed in the DNA repair test.
Mutat. Res. (1993) 290(2) 175-82 [RRC reference]
Last update 2017-11-23
Research papers using this strain
[Please submit your publication]
Graf U, Wurgler FE, Katz AJ, Frei H, Juon H, Hall CB, Kale PG.
Somatic mutation and recombination test in Drosophila melanogaster.
Environ Mutagen (1984) 6(2) 153-88 [PubMed ID = 6423380] [RRC reference]

Inoue H, Baba H, Awano K, Yoshikawa K.
Genotoxic effect of griseofulvin in somatic cells of Drosophila melanogaster.
Mutat. Res. (1995) 343(4) 229-34 [PubMed ID = 7623877] [RRC reference]

Fujikawa K, Fort FL, Samejima K, Sakamoto Y.
Genotoxic potency in Drosophila melanogaster of selected aromatic amines and polycyclic aromatic hydrocarbons as assayed in the DNA repair test.
Mutat. Res. (1993) 290(2) 175-82 [PubMed ID = 7694108] [RRC reference]
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