Detailed Information [109952]
Strain Information
DGRC Number 109952
Genotype with FlyBase Link w[1118] PBac{EGFP-IV}CanA-14F[KM0002]
Genotype w1118 PBac{EGFP-IV}CanA-14FKM0002
Break points/Insertion Site 14F1
Related Genes CanA-14F
Received Date 31 March 2014
Original Number KM0002
Chromosome X
Original Source Hiroshi Matsubayashi and Masa-Toshi Yamamoto, Kyoto Institute of Technology
Original Comments Insertion site: X: 16474227 (based on Drosophila melanogaster genome annotation release 5)
Cytological map: 14F1
Inserted gene: CanA-14F
Protein trap?: No
Insertion into Intron or Exon?: Intron (5'UTR)
Orientation of piggyTrap (F:Forward/R:Reverse): F
Orientation of GFP exon and its frame against inserted gene: Same orientation and frame, but premature stop
General Information Kyoto_piggyTrap
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Last update 2024-01-15
Research papers using this strain
[Please submit your publication]		 Tann JY, Xu F, Kimura M, Moore AW.
Mosaic Analysis with a Repressible Cell Marker (MARCM) Clone Generation in Drosophila Dendritic Arborization Neurons.
Cold Spring Harb Protoc (2023) [PubMed ID = 38148170] [RRC reference]

Library & Clone Information
Insertion Point X: 16474227
Chromosome Band 14F1
Flanking Sequence 
GTGGGGGGCCATTTTGCNGCAATAAANACCTCNATATACAGACCGATAAAACNNNNGCNT
CATTTTTNCGCANGATTATCTTTAACGTACGTCNCANTANGATTANCTTTCTGGGGGTTA
AAGTATTGAAATTAGTATTGCTTTGCTATTAAAANGGTATTATTAAAANNGTTTTTAATT
TCTTCTTAATATNCCNGAATTTGATCACAATAANCAAATGTTTCTTTCCGNGCATCTCNT
ATTATTATGTATCTGTGTCTGTTTGTGTGTATACGGANTTTAANGCNTGTNNCACCAAAA
ANCCAAATAAGTATGCTTTATCGANNCCCCAGCACAGTGACACACAGCACACACACACAC
ACACANCCCCCCCCNCCAACACNCTCATACANGTGTTGAA
Sequence Comment iPCR (3' sequence). Insertion point based on Drosophila melanogaster genome annotation release 5.

Insertion Point X: 16474227
Flanking Sequence 
ATTTGGGGGCCCTTTTCCANCTATCACCTAGGGTTAATACCTTATGATGCAAATAATAAT
AATAATAANAGTTGGCATTAAGGGCATCTTCCGCAGTGAATTCGGATTTAAGAAGTACTG
CTCCATTGTTTGAGAGAAATTTATTGGTGATTTTAATACCACAGTGATTTCTGTTGAAAT
GTAATTATTTAATTGACCTGCGCACTTGTCAGCATAGCTTTTCTTTCCGTGTGAGCAGTG
GGCACGGTGTGTCATATTTTAGCATTATATGCTCGGCCAACCCACCGCACATAACCTCAA
ACCGCGTTATGGCTGCGCCATCAAAAGCTCCGCTGTCCTGCTCTCTCTCCTTCTCTCTCT
CTCTCTTTCTCTTTCTCTGTCTCTGTCTATCTCTCTTTTT
Sequence Comment iPCR (5' sequence). Insertion point based on Drosophila melanogaster genome annotation release 5.

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