Detailed Information [109953]
Strain Information
DGRC Number 109953
Genotype with FlyBase Link w[1118]; PBac{EGFP-IV}PDCD-5[KM0048]
Genotype w1118; PBac{EGFP-IV}PDCD-5KM0048
Break points/Insertion Site 72D10
Related Genes PDCD-5
Received Date 31 March 2014
Original Number KM0048
Chromosome 3L
Comments Sb[1], TM6C may be segregating. 22July2020 T.O.
Original Source Hiroshi Matsubayashi and Masa-Toshi Yamamoto, Kyoto Institute of Technology
Original Comments Insertion site: 3L:16224734 (based on Drosophila melanogaster genome annotation release 5)
Cytological map: 72D10
Inserted gene: PDCD-5
Protein trap?: Yes
Insertion into Intron or Exon?: Intron (CDS)
Orientation of piggyTrap (F:Forward/R:Reverse): F
Orientation of GFP exon and its frame against inserted gene: Same orientation and frame
General Information Kyoto_piggyTrap
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Last update 2020-07-22
Research papers using this strain
[Please submit your publication]		 Tann JY, Xu F, Kimura M, Moore AW.
Mosaic Analysis with a Repressible Cell Marker (MARCM) Clone Generation in Drosophila Dendritic Arborization Neurons.
Cold Spring Harb Protoc (2023) [PubMed ID = 38148170] [RRC reference]

Library & Clone Information
Insertion Point 3L:16224734
Chromosome Band 72D10
Flanking Sequence 
CCTCCTTGGCCCCTTTTGANTATCTGNAAGNCAAGAAAAAATTAGCTGCAGATAAATAAA
CCTCGATATACAGACCGATAAAACACATGCGTCAATTTTACGCATGATTATCTTTAACGT
ACGTCGCAATATGATTATCTTTCTAGGGTTAAAGAAACAGGTGTCCCAGTCGAGAAGGAT
GACTCATAGGGAAGGTGTCTATAAGAACTAACAAAGCATACCATTGCTAATGTATTCTTA
ACATTTTTATTATAT
Sequence Comment iPCR (3' sequence). Insertion point based on Drosophila melanogaster genome annotation release 5.

Insertion Point 3L:16224734
Flanking Sequence 
TTTGNGTGGGNGGGNNATANTTGGCNGNAATAAATNNCCTCNATATACAGACCGATAAAA
CNGNNGCNTCATTNTTNCGCANGATTATCTTTAACGTACGTCGCANTANGATTANCTTTC
TNGGGTTAAAGAANCNAGGTGTCCCAGTCGANANAGGATGACTCAGGAGGGAAGGTGTCT
ATAAAAACNAACAAANCATACCNTTGCTAGNGTATTCTTAACNTTTTTATTATATTTTAT
ATAGTCTANTTTGCTTTCATTCCAAGTTANGACTATTTTNGGANTTCTCTAGAGGAATCT
ACCCGGTTNGGACCCACTCANATATTTCNNTTACAANTTGCNACTGCNTTTGGGACATGC
CCTCCNGGAACGTACCCTTCGGGCANGGCGGATTAAAAAAAANNTTCTTAATAAAA
Sequence Comment iPCR (3' sequence). Insertion point based on Drosophila melanogaster genome annotation release 5.

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