Detailed Information [109954]
Strain Information
DGRC Number 109954
Genotype with FlyBase Link w[1118]; PBac{EGFP-IV}Not1[KM0054]
Genotype w1118; PBac{EGFP-IV}Not1KM0054
Break points/Insertion Site 45F4
Related Genes Not1
Received Date 31 March 2014
Original Number KM0054
Chromosome 2R
Comments Cy, SM6a floating. 25Apr2017 T.O.
Original Source Hiroshi Matsubayashi and Masa-Toshi Yamamoto, Kyoto Institute of Technology
Original Comments Insertion site: 2R:5464623 (based on Drosophila melanogaster genome annotation release 5)
Cytological map: 45F4
Inserted gene: Not1
Protein trap?: Yes
Insertion into Intron or Exon?: Intron (CDS or 5'UTR)
Orientation of piggyTrap (F:Forward/R:Reverse): F
Orientation of GFP exon and its frame against inserted gene: Same orientation and frame
General Information Kyoto_piggyTrap
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]		 Tann JY, Xu F, Kimura M, Moore AW.
Mosaic Analysis with a Repressible Cell Marker (MARCM) Clone Generation in Drosophila Dendritic Arborization Neurons.
Cold Spring Harb Protoc (2023) [PubMed ID = 38148170] [RRC reference]

Library & Clone Information
Insertion Point 2R:5464623
Chromosome Band 45F4
Flanking Sequence 
ANATTNGGNNATCTTNTGTTTCTGGAAGGNAGAAAAAATGAGCTGCAGATAAATAAACCT
CGATATACCGACCGATAAAACACATGCGTCAATTTTNCGCATGATTATCTTTAACGTACG
TCCGCAATATGATTATCTTTCTAGGGTTAAATGGCAGATATAGNATTGTNGANAATTTCA
AAGNNCNNGTANTNTNTTTATTTTTTTTTTNCNCCNNATTG
Sequence Comment iPCR (3' sequence). Insertion point based on Drosophila melanogaster genome annotation release 5.

Insertion Point 2R:5464623
Flanking Sequence 
TNGNGTGGGNGGGNAANANTTNCCNGTAATAAANNCCCTCGANATACNACCGATCACCGN
GGCNTCATTTTTNCGCANGATTATCTTTAACGTACGTCNCAGTANGATTANCTTTCTNGG
GTTAAGNGGCANATATNTGATNGTTGAGAATTTCAAAATACTGAAGTATANGACTCTGTT
TTGNTATCATTTTTTTTTTAACCNTTTTTTTNANTTTNGNNNNAAAANNCCTTTTCNGGN
AAAAAAAAGGGGAANCNGAANNANNTTTNNCCCCNNAAACAANTTNTTTTTNNGGGNNTN
NTNGGGAAANGGGNCCCTTTCAAACCNANTTTTTTNTTAAAAACCNATTTGGAGGGGNAA
ATTTTTTNGGGGGGGCAAAAANNCCCCCCCCCCCCCNAAGGGAACCNAAANNNGTNAAAA
NNTTTGAACCNGGAACCTNGAANNCNGAAAANCAGGNCCNCCCCCCAAAGGGGGGAACCA
AAAACANCTNTTTNGNCCCCCCCTNCNAATNTNCNCNAAAAAAANTNCCCCCCCCCCCCN
GGAACTNNCCNTNCGGGNNNGGNGATNAAAAAAAA
Sequence Comment iPCR (3' sequence). Insertion point based on Drosophila melanogaster genome annotation release 5.

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