Detailed Information [109955]
Strain Information
DGRC Number 109955
Genotype with FlyBase Link w[1118]; PBac{EGFP-IV}KM0129
Genotype w1118; PBac{EGFP-IV}KM0129
Break points/Insertion Site 102A6
Received Date 31 March 2014
Original Number KM0129
Chromosome 4
Original Source Hiroshi Matsubayashi and Masa-Toshi Yamamoto, Kyoto Institute of Technology
Original Comments No annotated gene.
Insertion site: 4: 202199 (based on Drosophila melanogaster genome annotation release 5)
Cytological map: 102A6
Inserted gene:
Protein trap?:
Insertion into Intron or Exon?:
Orientation of piggyTrap (F:Forward/R:Reverse): R
Orientation of GFP exon and its frame against inserted gene:
General Information Kyoto_piggyTrap
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]		 Tann JY, Xu F, Kimura M, Moore AW.
Mosaic Analysis with a Repressible Cell Marker (MARCM) Clone Generation in Drosophila Dendritic Arborization Neurons.
Cold Spring Harb Protoc (2023) [PubMed ID = 38148170] [RRC reference]

Library & Clone Information
Insertion Point 4: 202199
Chromosome Band 102A6
Flanking Sequence 
CCGGGCCCCGTGGTAAGATAAGAGGATACCGCCCCGGTGAAAGCTCCTCGCCCTTGCTCA
CCATTTTGATTATCTGTAAGGAAGAAAAAATTAGCTGCAGATAAATAAACCTCGATATAC
AGACCGATAAAACACATGCGTCAATTTTACGCATGATTATCTTTAACGTACGTCGCAATA
TGATTATCTTTCTAGGGTTAAGGTACATTTTTCGTAATACATTGTTGTATACATAATATA
AATAATATATTTAGCTTTAAGGTTAATCGGACATGTGGACCCCATATTCCAACTCAACTA
AAAAGAAAGTGGCCCTAACTCCGAAGGACAACAAGACGCATTTTATATTTATTTGTAATA
TTATTTAAATAATAAACAAAATTTAAAAAAAAAAATTATCACTTCAAAAAGGGGTTAGGA
TTAAATGAATAAACAGCGGGGAAAAAGCGAAAAATACCCCGATGATTTACCGTTTTAAAT
ACGCTTAAAAAGCGGTGATTAAAAGCGATGATTTTGCGCCCCTGGACGTAGCCTTCGGGC
ATGGCGGACTTGAAAAAGTCGGGCTGCTTCTTGGGGTCGGGGTAGCGA
Sequence Comment iPCR (3' sequence). Insertion point based on Drosophila melanogaster genome annotation release 5.

Insertion Point 4: 202199
Flanking Sequence 
NTTTGNGTGGGGGGAANANTTGNCNGTAATANNNCCCTCGATATCAGACCGATCACCGNG
GCNTCATTTTTNCGCANGATTATCTTTAACGTACGTCNCAGTANGATTANCTTTCNGNGG
GTTAAGGTACTTTTTTCGAAATACTTTGTTGTATNCATAATATAAATAATATATTTNGCT
TTANGGTTAANCGGACGTGNGGACCCCATATTCCAACTCAACNAAAAAGANAGNGGCCCT
ANCTCCNANGGAACAACAAGACGCATTTTATATTTATTTGNAATNTTATTTAAATAATAA
ACNAANTTTAAAAAAAAAATTTNNCNNTTNAAANGGGGGTTGGGAATTAANNGAANANCC
NGNGGTGAAAAAANCGAAAAAACNCCCCGNTGAATTTCCCGTTTNAAANNCCCTAAAAAA
NGNGGNGAANAAAAANCAAATNNTTTTNCCCNCCGGGAACNTACCNTTCGGGCNGGGNGA
TTAAAAAAANNNTTTTTTANAAA
Sequence Comment iPCR (3' sequence). Insertion point based on Drosophila melanogaster genome annotation release 5.

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