Detailed Information [109959]
Strain Information
DGRC Number 109959
Genotype with FlyBase Link w[1118]; PBac{EGFP-IV}Snap25[KM0227] / TM6C, Sb
Genotype w1118; PBac{EGFP-IV}Snap25KM0227 / TM6C, Sb
Related Genes Snap25
Received Date 31 March 2014
Original Number KM0227
Chromosome 3L
Original Source Hiroshi Matsubayashi and Masa-Toshi Yamamoto, Kyoto Institute of Technology
Original Comments The insertion into the multicopy transposon (Stalker). 3'RACE and sequence analyses indicated that the SNAP-25 gene is trapped. Maybe cold paralysis.
Insertion site: 3L (based on Drosophila melanogaster genome annotation release 5)
Cytological map:
Inserted gene: Snap25
Protein trap?: Yes?
Insertion into Intron or Exon?:
Orientation of piggyTrap (F:Forward/R:Reverse):
Orientation of GFP exon and its frame against inserted gene:
General Information Kyoto_piggyTrap
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Lethality lethal
Last update 2023-11-27
Research papers using this strain
[Please submit your publication]

Library & Clone Information
Insertion Point 3L
Flanking Sequence 
CATCGGGGCACATTTTGGGCACATTTGATTTCTGTAAGGAAGAAAAAATTAGCTGCAGAT
AAATAAACCTCGATATACAGACCGATAAAACACATGCGTCAATTTTACGCATGATTATCT
TTAACGTACGTCGCAATATGATTATCTTTCTAGGGTTAAAGTATTGTGCTAATAATTAAA
GTCGACTGATCTTAGTTCGACTATAAATTAAATAAATTTATAAATTCAAATAACATTATA
AAACATAATAATTGATGGCGGAAGAGCCACAATTCTCAAACGCACAGCCCACGACACAAA
GGGACTTGCCAACGTTGGAAGAAGCTCTGCAGCAAAATCCTGCAGATGGACCACGCCCAC
TCACAATAGCTGAGTACCGGGCAAGGCAGAAAAGGAAGGAACCAAAGAAGCACAAACGCT
CCGGAAAAAGGGTAAAGCTACTTAAACAACGCCGACTGGTTAAGGAAATGACCCAGTTGG
CCAGAGACGAGGCATCCCGACAACGCTACAAAGAACGTCTTGAGGACATCGAAAGCAAGA
TTTCGCAAGGTGCGAAACAACGCAAACGGGCTGCATAAATAAATGCCAATGCCCCAATTT
GCCTTAATTTTAATTTCTAAACCCAAGCCGATGCGGGAAACCGCTGCTTGAAAAACACTA
ATATCTGTTAGGCTTTTTACTAACAATGCGGTTGGACAATTTTTTTTTATAATGTAAACA
AATATGTAAGCCAACCACATTTATTAACAACTAATATTTCCACGTCTCCGCTACTGAGTA
TATATATATTCAGCTGCAAAAAATTTTTTTTTAATGACAGAATATAACAATTTCTTATCT
TCATTAAACGTAAACATTTTTTTTTTATTTTTTGGATCTCTCTAGGAGGAGTAACTTAAT
ATCATACTACTGAGACACAACTCGTAGTAGAAGAAAGAAGACGCGTTTCCTGTGTGAAAT
TAAACCCGA
Sequence Comment iPCR (3' sequence). Insertion point based on Drosophila melanogaster genome annotation release 5.

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