Detailed Information [109971]
Strain Information
DGRC Number 109971
Genotype with FlyBase Link w[1118]; PBac{EGFP-IV}Pax[KM0601]
Genotype w1118; PBac{EGFP-IV}PaxKM0601
Break points/Insertion Site 37D6
Related Genes Pax
Received Date 31 March 2014
Original Number KM0601
Chromosome 2L
Comments Expression Photo: KM0601
Original Source Hiroshi Matsubayashi, Kyoto Institute of Technology
Original Comments Insertion site: 2L: 19405553 (based on Drosophila melanogaster genome annotation release 5)
Cytological map: 37D6
Inserted gene: Pax
Protein trap?: Yes
Insertion into Intron or Exon?: Intron (CDS)
Orientation of piggyTrap (F:Forward/R:Reverse): F
Orientation of GFP exon and its frame against inserted gene: Same orientation and frame
General Information Kyoto_piggyTrap
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]		 Sherrard KM, Cetera M, Horne-Badovinac S.
DAAM mediates the assembly of long-lived, treadmilling stress fibers in collectively migrating epithelial cells in Drosophila.
Elife (2021) 10 [PubMed ID = 34812144] [RRC reference]

Library & Clone Information
Insertion Point 2L: 19405553
Chromosome Band 37D6
Flanking Sequence 
NGTGGGNGNGAGNNTTTNCNGNAATAANNCCTCGATATCAGACCGATAACCANNNGCNTC
ATTTTTNCNCANGATTATCTTTAACGTACGTCNCATTATGATTANCTTTCNGGGGGTTAA
NCCTTAANCCTTTATAAAAATTANCATTTATTAAATAGNGCCNATCAATATTTAGGGCAT
TGCATTAAAGACNGGCANTTGATGTGGNGGGTAATATTTTCACNTTCCTTCAAATCATCG
TACTACTTATTAAANTGTTGTANCCNCNNGGAAANGACTCTNTCACTGNGGACAGTACTC
TGCGTAGTGNCGAANCGCNCCNTTTATTAAAATATTGTTTATATNCCAAATTAAATCGAC
CCTTCCCAAAATCTTATAGTCAGAGCATTCCATTTTGGTTGAATCAGGGCAANCCTTANT
GTTATTAAGATTTATATATCCCCCCCGCCNTTTCAAACAAAACTCCGCTCCTGTTTTCGC
CTGCCGCAAAAATTTTCATTGGCTTCAGCCGTCACCGAAGGTTTTCTTATTCCAAATATT
TTCNTTGCCTATTNAAAGACCCAAATCNTACCNCAGCGCTCCNGGACNTACCCTTCGGGC
ANGCGATTAAAAAAAAAAANNCNNCC
Sequence Comment iPCR (3' sequence). Insertion point based on Drosophila melanogaster genome annotation release 5.

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