Detailed Information [140323]
 

Strain Information
DGRC Number 140323
Genotype with FlyBase Link y[*] w[*]; PBac{SAstopDsRed}LL01328 P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; PBac{SAstopDsRed}LL01328 P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 66A19
Related Genes CG32368, CG8111
Received Date 19 October 2007
Original Number LL01328
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3L:7908920(+)
Cytological Band: 66A19
Gene Symbol-1: CG32368
CG Number-1: CG32368
FlyBase ID-1: FBgn0052368
Insertion Type-1: 3' UTR
Gene Symbol-2: CG8111
CG Number-2: CG8111
FlyBase ID-2: FBgn0035825
Insertion Type-2: Putative Promoter.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
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Library & Clone Information
Strand Plus
Insertion Point 7908920
Chromosome Band 3L
Flanking Sequence 
tttacgcagactatctttctagggttaaAATGCCTTATTAATAAATTTCATGAACAAAAC
AAATTGCTGTACACATTATTTGGTAAAAGACTACACATTAGCATGGGATATAGTCKAWTT
MACSAWWGTGGKAACACTARAACTAGCTMGTTCTAGAGCGGCSGCCACCSCGKTGGAGCT
CCRATTCRCCCTAWWSTGAGTCGTAWTAYRTTATCTAGTTAGGCGCGCCTGTGGGACGGA
AGAACAKAWKAATTARATWTCTATRACAAKAAMATAWATRTATAATAAGTTATCACGTAA
GTAGAASATGAAATAACAATATAATTATCGTATGAGTTAAATCTTAAAAGTCACGTAAAA
GATAATCATGCGTCATTTTGACTCACGCGGTCGTTATAGTTCAAAATCAGTGACACTTAC
CGCATTGACAAGCAAGTCACARATGAYCTMAMAGCWTTTAGTMMGATGTCKTGAWTGAAS
TRCAGCGGGYTTTTAGGATCAGTGGTGGYKTMCTCAGCGGTTGTCAGATCGGCGCTGGGY
GMMTATAAACTCGGAAGAACCCGAGGCGGAAGGA
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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