Detailed Information [141186]
 

Strain Information
DGRC Number 141186
Genotype with FlyBase Link y[*] w[*]; PBac{SAstopDsRed}LL04152 P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; PBac{SAstopDsRed}LL04152 P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 73B1
Related Genes CG32164, CG18217
Received Date 9 November 2007
Original Number LL04152
Chromosome 3
Comments No Tb expression but Hu is OK. 07Jun2023 E.O.
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3L:16596702(+)
Cytological Band: 73B1
Gene Symbol-1: CG32164
CG Number-1: CG32164
FlyBase ID-1: FBgn0042177
Insertion Type-1: Intron
Gene Symbol-2: CG18217
CG Number-2: CG18217
FlyBase ID-2: FBgn0036646
Insertion Type-2: Putative Promoter.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2024-04-25
Research papers using this strain
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Library & Clone Information
Strand Plus
Insertion Point 16596702
Chromosome Band 3L
Flanking Sequence 
tttacgcagactatctttctagggttaaTTAATATTATATTTTTATTTGCATTAAATTCG
TGGATATACCATTACGAACATcgaattaaccattgtgggaacactagaac
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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