Detailed Information [109700]
 

Strain Information
DGRC Number 109700
Genotype with FlyBase Link w[1118]; PBac{EGFP-IV}eIF4G[KM0133]
Genotype w1118; PBac{EGFP-IV}eIF4GKM0133
Break points/Insertion Site 102F1
Related Genes eIF4G
Received Date 22 November 2012
Original Number 133
Chromosome 4
Original Source Hiroshi Matsubayashi and Masa-Toshi Yamamoto, Kyoto Institute of Technology
Original Comments Insertion site: 4:950257 (based on Drosophila melanogaster genome annotation release 5)
Cytological map: 102F1
Inserted gene: eIF4G
Protein trap?: Yes
Insertion into Intron or Exon?: Intron (CDS)
Orientation of piggyTrap (F:Forward/R:Reverse): F
Orientation of GFP exon and its frame against inserted gene: same orientation and frame
General Information Kyoto_piggyTrap
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]		 Kuklin EA, Alkins S, Bakthavachalu B, Genco MC, Sudhakaran I, Raghavan KV, Ramaswami M, Griffith LC.
The Long 3'UTR mRNA of CaMKII Is Essential for Translation-Dependent Plasticity of Spontaneous Release in Drosophila melanogaster.
J Neurosci (2017) 37(44) 10554-10566 [PubMed ID = 28954869] [RRC reference]
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Library & Clone Information
Insertion Point 4:950257
Chromosome Band 102F1
Flanking Sequence 
ATACCCCTTTTGGATTTTCTGTAAGGAGAAAAAATTAGCTGCAGATAAATAAACCTCGAT
ATACAGACCGATAAAACACATGCGTCAATTTTACGCATGATTATCTTTAACGTACGTCGC
AATATGATTATCTTTCTAGGGTTAATAGATTGTTTACATTGACTACAGCAGGCAAACTTA
TAAAAAATAGGTTTTAGCTCTTTTCGGAACTAGGAAAACATATTTATTTAACGAACTAAT
TTAAAACAATTGCAAGGAAAGTAATACAGCAAATAATGTCAAAATGTATATATGTAAATA
ATTTTGGAGATGTTATTTTTATATCGACTTACCATATTTTGTGCTGAATGGGGCTGCATG
GCTTTATCTATATCTGATTGTGTTGGTAAAGTTGGTATAGCCTGTTGCATTACGTATGTG
TATTCCTAGAGTTAGCTCTGTAGGAAATCGCCAAACGGTTAGATTTACCGTTTCATAGAC
GATTTCTGTGGTGATTTTGGACCCACAAATACGACGTCATTCAATCATTTGATTCTTTAA
TGAATATTAATGAATATTAATTGTCCACGTTTCAGTTCTGCATATTTTACCTGGAAATAT
ATTGATATACAGCTACCAGAGAGGTCTCTGAAATTAAAAGAACTGACACGTATAAAATCA
TTAAATATCACAAAAGATTTCACCCAAATGTTTCAAAAATATAAAGTAGACATTTTGTAA
TTTATTAAGTCTAGAGCTGAAGCCCAGAGATAATTTCTATTAAACATTTGCCAATCCATC
CTGGAGCCTTACTTATTTGCCGACGTTGCCGTTGTGACTTTGGAGATTTCGTTCTCACAA
TCACATGTGCATATGGTGAAACGTGCCACGACTTCATCGTTTACATTATTTTTTAAAAGT
TAAGACCACAATTTCAGATGTTTA
Sequence Comment iPCR (3' sequence). Insertion point based on Drosophila melanogaster genome annotation release 5.

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