Detailed Information [115362]
 

Strain Information
DGRC Number 115362
Genotype with FlyBase Link w[1118]; PBac{544.SVS-1}14-3-3epsilon[CPTI100034]
Genotype w1118; PBac{544.SVS-1}14-3-3εCPTI100034
Break points/Insertion Site 90F10
Received Date 03 December 2009
Original Number 100034
Chromosome 3
Comments Flannotator: CPTI-100034

FlyBase Insertion: PBac{544.SVS-1}14-3-3epsilon[CPTI100034]

Also known as CPTI-100034
Original Source Department of Genetics, University of Cambridge
Original Comments Location: 14068843
Chromosome: 3R
Gene CG: CG31196
Gene Name: 14-3-3epsilon
Gene Feature: intron_CG31196:1_CG31196:2
YFP Status: Confirmed - Yes
In frame: Yes
Donor: 544 KH3.1B
Jumps from: ----

Stock report in FlyProt
Element name:CPTI-100034Line ID: 1Eye colour:----
Donor:544 KH3.1B (Males)Construct:PBac{SVS-1}Lethality:homozygous lethal
YFP expression?YesBalancer:TM2Chromosome:3
Notes:
High level of fluorescence during the whole of embryogenesis. Present in freshly laid eggs, then ubiquitous expression until the end of embryogenesis with a pic of strong expression at stage 11. Expressed in pole cells. Strongly expressed in central
nervous system at stage 17. Same location as 100045

General Information CPTI
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Lowe N, Rees JS, Roote J, Ryder E, Armean IM, Johnson G, Drummond E, Spriggs H, Drummond J, Magbanua JP, Naylor H, Sanson B, Bastock R, Huelsmann S, Trovisco V, Landgraf M, Knowles-Barley S, Armstrong JD, White-Cooper H, Hansen C, Phillips RG, UK Drosophila Protein Trap Screening Consortium, Lilley KS, Russell S, St Johnston D.
Analysis of the expression patterns, subcellular localisations and interaction partners of Drosophila proteins using a pigP protein trap library.
Development (2014) 141(20) 3994-4005 [RRC reference]

Lye CM, Naylor HW, Sanson B.
Subcellular localisations of the CPTI collection of YFP-tagged proteins in Drosophila embryos.
Development (2014) 141(20) 4006-17 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]
Lye CM, Naylor HW, Sanson B.
Subcellular localisations of the CPTI collection of YFP-tagged proteins in Drosophila embryos.
Development (2014) 141(20) 4006-17 [PubMed ID = 25294944] [RRC reference]

Lowe N, Rees JS, Roote J, Ryder E, Armean IM, Johnson G, Drummond E, Spriggs H, Drummond J, Magbanua JP, Naylor H, Sanson B, Bastock R, Huelsmann S, Trovisco V, Landgraf M, Knowles-Barley S, Armstrong JD, White-Cooper H, Hansen C, Phillips RG, UK Drosophila Protein Trap Screening Consortium, Lilley KS, Russell S, St Johnston D.
Analysis of the expression patterns, subcellular localisations and interaction partners of Drosophila proteins using a pigP protein trap library.
Development (2014) 141(20) 3994-4005 [PubMed ID = 25294943] [RRC reference]
Stock Request

Library & Clone Information
Library Name / Clone Name PBac{544.SVS-1}14-3-3epsilon[CPTI100034]
Strand Minus
Insertion Point 3R:14068843..14068843
Chromosome Band 3R
Sequence Comment Data from FlyBase (insertion_mapping_fb_2011_09.tsv)

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