Detailed Information [140455]
 

Strain Information
DGRC Number 140455
Genotype with FlyBase Link y[*] w[*]; P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B PBac{SAstopDsRed}LL01754 P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B PBac{SAstopDsRed}LL01754 P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 84e5
Related Genes Taf7 (CG2670)
Received Date 19 October 2007
Original Number LL01754
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: ~3R:3733649(+)
Cytological Band: 84e5
Gene Symbol-1: Taf7
CG Number-1: CG2670
FlyBase ID-1: FBgn0024909
Insertion Type-1: CDS
Gene Symbol-2:
CG Number-2:
FlyBase ID-2:
Insertion Type-2:
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Lethality lethal
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]		 Neves A, Eisenman RN.
Distinct gene-selective roles for a network of core promoter factors in Drosophila neural stem cell identity.
Biol Open (2019) 8(4) [PubMed ID = 30948355] [RRC reference]
Stock Request

Library & Clone Information
Strand Plus
Insertion Point 3733649
Chromosome Band 3R
Flanking Sequence 
NNNNNNNNNNNNNNNNGNNGTNANNNNTNACNGCNGACTATNTTTCATAGGGTTAAGGAC
CGGCTGACCATCCAACTGGATCCGGATCTGAGGTACGGTGAAGTGCGAATAGATGATCAG
ATACTGTACACGAAACTCGTGGATTTGCCCACCGTCGTCGAATTAACCNTTGTGGGAACA
CTAGAACTAGCTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAATTCGCCCTATAG
TGAGTCGTATTACGTTATCTAGTTAGGCGCGCCTGTGGGACGGAAGAACAGATGAATTAG
ATATCTATAACAAGAAAATATATATATAATAAGTTATCACGTAAGTAGAANATGAAATAA
CAATATAATTATCGTATGAGTTAAATCTTAAAAGTCACGTAAAAGATNN
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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