Detailed Information [140754]
 

Strain Information
DGRC Number 140754
Genotype with FlyBase Link y[*] w[*]; P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B PBac{SAstopDsRed}LL03310 P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B PBac{SAstopDsRed}LL03310 P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 85D1
Related Genes D1 (CG9745)
Received Date 25 October 2007
Original Number LL03310
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: ~3R:5065246(+)
Cytological Band: 85D1
Gene Symbol-1: D1
CG Number-1: CG9745
FlyBase ID-1: FBgn0000412
Insertion Type-1: CDS
Gene Symbol-2:
CG Number-2:
FlyBase ID-2:
Insertion Type-2:
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Lethality semi lethal
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]		 Jagannathan M, Yamashita YM.
Defective Satellite DNA Clustering into Chromocenters Underlies Hybrid Incompatibility in Drosophila.
Mol Biol Evol (2021) 38(11) 4977-4986 [PubMed ID = 34302471] [RRC reference]

Jagannathan M, Cummings R, Yamashita YM.
A conserved function for pericentromeric satellite DNA.
Elife (2018) 7 [PubMed ID = 29578410] [RRC reference]

Jagannathan M, Cummings R, Yamashita YM.
The modular mechanism of chromocenter formation in Drosophila.
Elife (2019) 8 [PubMed ID = 30741633] [RRC reference]
Stock Request

Library & Clone Information
Strand Plus
Insertion Point 5065246
Chromosome Band 3R
Flanking Sequence 
GAGATAAGGTGTTATTAGGGCCTGCCGGCCTTGCGCTTCTTGGCGGAACCGGGCGTCTTC
ACCGAATCCCCGGATCCTGATCCGGCGCCACCACTGCTCTTGGGTCGTCCCCTGCCCTTC
GTCGGCGAGGGTGATGAGTTGTTTGCAAGCAAAGCGCCGGAGCCATCGCCATCGCCGTCC
TCCCCCCCCTCGTCTTCGGGATCTTCGTCATTTTGGATTTTTGACGCCTTCGGTGGGCGA
CCCCGCTGTCCACCACCGCCACTGGAGCCCTTGTTCGTGGCGGGACGACCGCGCTTTTAA
ATGCCTGGCGAGATTGCTGCAACCGCCGCTGTTGAAGATTTACCACCGACGGATGCCTTT
GACGGTCGGACGCGCTTCATTACCGCGACTTCCTCCATTTCTCTCGGTCGGATTCAATTA
GCAAGCGAATAAAAAAAATCCTTGTTTCTTCACGTCCCCTCACACACACACGCGCACAAA
TGCTCTCTCTCACGAACACACGCTCACTCTTTTCGCTTGGCTCCGCTCTTCTTCTTCACC
TTTTTGTTCGCCGAAATCAAGCTGGAAAAATATACAGAAAAACAATTTTGCATTAGGGCT
TGATGCGCATAATGCTGCAAAAAATATCCTCAAAAATTCCTT
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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