Detailed Information [141954]
 

Strain Information
DGRC Number 141954
Genotype with FlyBase Link y[*] w[*]; P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E PBac{SAstopDsRed}LL07056 / TM6B, Tb[1]
Genotype y* w*; P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E PBac{SAstopDsRed}LL07056 / TM6B, Tb1
Break points/Insertion Site 99D5
Related Genes sima (CG7951)
Received Date 11 December 2008
Original Number LL07056
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3R:25905350(+)
Cytological Band: 99D5
Gene Symbol-1: sima
CG Number-1: CG7951
FlyBase ID-1: FBgn0015542
Insertion Type-1: Intron
Gene Symbol-2:
CG Number-2:
FlyBase ID-2:
Insertion Type-2:
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]		 Lee B, Barretto EC, Grewal SS.
TORC1 modulation in adipose tissue is required for organismal adaptation to hypoxia in Drosophila.
Nat Commun (2019) 10(1) 1878 [PubMed ID = 31015407] [RRC reference]
Stock Request

Library & Clone Information
Strand Plus
Insertion Point 25905350
Chromosome Band 3R
Flanking Sequence 
tttacgcagactatctttctagggTTAATGACCAATCATTCACTATATTCCGACTATGTC
ATAGTATTACATTTGATGTAATCCTCGCAGTGCACGCCTGAAACTGACCCGGCCAAGGAT
TCACCTTACTAAGGACCTGCTGCACCTGCTCCTTTGGCAACTCTCGGTTCGTGCGCTGCG
TTGATGATGATTTACTGTTGTGTTGCTGCCAGGATTTGCCCGTGTACTCGAAttaaccat
tgtgggaacactagaac
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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