Detailed Information [109696]
 

Strain Information
DGRC Number 109696
Genotype with FlyBase Link w[1118]; PBac{EGFP-IV}RpS20[KM0175] / TM2
Genotype w1118; PBac{EGFP-IV}RpS20KM0175 / TM2
Break points/Insertion Site 93A1
Related Genes RpS20
Received Date 22 November 2012
Original Number 175
Chromosome 3R
Comments Expression Photo: KM0175
Original Source Hiroshi Matsubayashi and Masa-Toshi Yamamoto, Kyoto Institute of Technology
Original Comments Insertion site: 3R:16648115 (based on Drosophila melanogaster genome annotation release 5)
Cytological map: 93A1
Inserted gene: RpS20
Protein trap?: Yes
Insertion into Intron or Exon?: Intron (CDS)
Orientation of piggyTrap (F:Forward/R:Reverse): R
Orientation of GFP exon and its frame against inserted gene: same orientation and frame
General Information Kyoto_piggyTrap
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]		 Pulianmackal AJ, Kanakousaki K, Flegel K, Grushko OG, Gourley E, Rozich E, Buttitta LA.
Misregulation of Nucleoporins 98 and 96 leads to defects in protein synthesis that promote hallmarks of tumorigenesis.
Dis Model Mech (2022) 15(3) [PubMed ID = 35107131] [RRC reference]
Stock Request

Library & Clone Information
Insertion Point 3R:16648115
Chromosome Band 93A1
Flanking Sequence 
CTTAGATATCTGTAAGGAAGAAAAAATTAGCTGCAGATAAATAAACCTCGATATACAGAC
CGATAAAACACATGCGTCAATTTTACGCATGATTATCTTTAACGTACGTCGCAATATGAT
TATCTTTCTAGGGTTAATCGGGGTTAGGTTGAACGGCCATCGCAGGCGCCCGGGATTGCG
AGTTGGCGAGGGCATGGCTTCTTCTACTATTGACTCGCAGAGTGAGAGGATTGTTCGGAA
ATGACATTCTTACCATTATTTACAAATTTGCATGGCCAAGATGCTCAAATCTTTGTATTT
TGAGGGCATGTCCTGGATGAAGACTTCGGGCTGGTTCGACTGGCGGAGTAGTGCTGCTTC
ATGTGGCGGGGTAGCGAAAAACCGAGGTCCGTCTTGCACGCGCCTAAGGTTTGAGATTTC
CAAAGAACCGGGCCGTGGGGGAGGGGGCCCACATGAAGAGCACGACTTCTTCAACCGCGC
GGTGCAGACGGCTTCTTCAGGTCCGCCTGCCCGAAGGCTACGTCCAGGATCGCTGCTCCT
CTACAATTATTGCACTACTTTGACTCGGTTGCTCGCTGACTAATGGAAAGCAATGCAATC
TCGCAATCGTCGAACTATAGCTACACTCTAGTTGCTGCTCGATTGCCTTGCAGATAATAC
ATTGCGAGCAATGACAGATACTCACGCGTTAACAGACGAGAGATATATGCTCGTAGTTAA
GATTTACTGCGCGCGCTGAGTGTTCTTCTTACGATTATATATGGGGTATATGATGTGCGA
TTGTTCTTCTTCTGATGCTCATAGAGGCTGACAGAGCTAGCACTGTTCCTGCTGATGCAT
CAGGATCACTGCACGCACTCTACACAACAAAGTCACTCTCGGGGAGAGAGACCCCACCCC
CTCTACTTAATATAAA
Sequence Comment iPCR (3' sequence). Insertion point based on Drosophila melanogaster genome annotation release 5.

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