Detailed Information [109722]
 

Strain Information
DGRC Number 109722
Genotype with FlyBase Link w[1118]; PBac{EGFP-IV}brat[KM0072] / SM6a
Genotype w1118; PBac{EGFP-IV}bratKM0072 / SM6a
Break points/Insertion Site 37C5
Related Genes brat
Received Date 22 November 2012
Original Number 72
Chromosome 2L
Comments Expression Photo: KM0072
Original Source Hiroshi Matsubayashi and Masa-Toshi Yamamoto, Kyoto Institute of Technology
Original Comments Insertion site: 2L:19162806 (based on Drosophila melanogaster genome annotation release 5)
Cytological map: 37C5
Inserted gene: brat
Protein trap?: No
Insertion into Intron or Exon?: Intron (5'UTR)
Orientation of piggyTrap (F:Forward/R:Reverse): R
Orientation of GFP exon and its frame against inserted gene: same orientation but wrong frame (5'UTR intron)
General Information Kyoto_piggyTrap
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Lethality lethal
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]
Stock Request

Library & Clone Information
Insertion Point 2L:19162806
Chromosome Band 37C5
Flanking Sequence 
TTGCTTGCCCCNCNTGATTCTNAAGGAAGAAAAAATTAGCTGCAGATAAATAAACCTCGA
TATACAGACCGATAAAACACATGCGTCAATTTTACGCATGATTATCTTTAACGTACGTCG
CAATATGATTATCTTTCTAGGGTTAAACATCATTCAGTGATTTAGTCCCAATATTCTGAC
CGCAACTTTATGTCGAGGATACAGACGTACTCAAATGCAGCGAAAAGTAAAAGAAGCCCA
GCACAAAGAGCAAGC
Sequence Comment iPCR (3' sequence). Insertion point based on Drosophila melanogaster genome annotation release 5.

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