| Strain Information | |
|---|---|
| DGRC Number | 117681 |
| Genotype with FlyBase Link | P{w[+mC]=Hsp83-Osat\SPO11D.Y213F}M33-F1, w{*] / Binsinscy |
| Genotype | P{w+mC=Hsp83-Osat\SPO11D.Y213F}M33-F1, w{* / Binsinscy |
| Received Date | 13 March 2014 |
| Original Number | F353 |
| Chromosome | 1 |
| Also known as | w P{w+, hsp83pro-OsSPO11D[Y213F]-hsp70TT M33-F1}/Binsinsky |
| Original Source | Kohji Kusano, Kyoto Institute of Technology |
| Original Comments | This transgene is on the X chromosome. The base change causing Y213F amino acid change was introduced into a cDNA of Oryza sativa OsSPO11D gene (a homologue of D. melanogaster mei-W68 gene) cloned into a P-element transformation vector CaSpeR-hsp83, which includes hsp80 promoter and hsp70 terminator as well as a mini-white gene, and transformed. The ectopic expression from this transgene did not show double-stranded break-forming activity. |
| Genus | Drosophila |
| Subgenus | Sophophora |
| Species Group | melanogaster |
| Species Subgroup | melanogaster |
| Species | melanogaster |
| Reference | Shingu Y, Tokai T, Agawa Y, Toyota K, Ahamed S, Kawagishi-Kobayashi M, Komatsu A, Mikawa T, Yamamoto MT, Wakasa K, Shibata T, Kusano K. The double-stranded break-forming activity of plant SPO11s and a novel rice SPO11 revealed by a Drosophila bioassay. BMC Mol Biol (2012) 13 1 [RRC reference] |
| Last update | 2020-04-03 |
| Research papers using this strain [Please submit your publication] |
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