Detailed Information [140001]
Strain Information
DGRC Number 140001
Genotype with FlyBase Link y[*] w[*]; PBac{SAstopDsRed}LL00001 P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM3, Sb[1]
Genotype y* w*; PBac{SAstopDsRed}LL00001 P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E / TM3, Sb1
Break points/Insertion Site 71B1
Related Genes CG3020
Received Date 19 October 2007
Original Number LL00001
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3L:15013014(-)
Cytological Band: 71B1
Gene Symbol-1: CG3020
CG Number-1: CG3020
FlyBase ID-1: FBgn0036464
Insertion Type-1: Intron
Gene Symbol-2:
CG Number-2:
FlyBase ID-2:
Insertion Type-2:
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]		 Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [PubMed ID = 18267091] [RRC reference]

Library & Clone Information
Strand Minus
Insertion Point 15013014
Chromosome Band 3L
Flanking Sequence 
NNNNNNNNNNGCATGCGTCATTTTACGCAGACTATCTTTCTAGGGTTAAGGTGAAAGTGA
GGTTTTCTTATGAGGATATGCAGAAATGGTTCACCATGACCTGCATGGGAATAATTAATG
CCGGTTCAGGGCTTCCAACCTGAAACCGCACTCGTGCCGGGCATTTCATCCTGTTCCATT
TCCTAGTCGGTGGTTAGTCCAATCTAATGGCGTGTGAGTGTATTTGCGTAAGCGGATTAA
CAGCGGTCAAATGTGTGGATGCACTTTCAGAAAAGTTACTTTATGTTCGCAGAATTACAA
ATGTTGCCATACGTATTGAATGCAAAAGTTCTATGGATTCATTTTGAAAATACTATTGTG
CTGCCTATTTTTTTTGACTATTAAAAATACAACATTACTATAAAAATCTATTTTCATCCG
GTACTGAAAGGGCTACTATTTCGGTTTATTTCTTTGAGTGTAAAGTGGGAGAGCAAATAA
GTGAAGCAGCAGTTTCTTTTCCCGCTAAAAGTCCAAGGTAGCCAAGCAACAAAACCTTCT
ATTTACCGCAGAGAAGAAAAGCAGAGTTCCCATAACCAAAGTCCCTCGAATTAACCATTG
TGGGAACACTAGAACTAGCTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAATTCG
CCCTATAGTGAGTCGTATTACGTTATCTAGTTAGGCGCGCCTGTGGGACGGAAGAACAGA
TGAATTANATATCTATAACAAGAAAATATATATATAATAAGTTATCACGTAAGTAGAACA
TGAAATAACAATATAATTATCGTATGAGTTAAATCTTAAAAGTCACGTAAAANATNATTC
ATGCGTCA
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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