Detailed Information [140003]
 

Strain Information
DGRC Number 140003
Genotype with FlyBase Link y[*] w[*]; P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E PBac{SAstopDsRed}LL00008 / TM3, Sb[1]
Genotype y* w*; P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E PBac{SAstopDsRed}LL00008 / TM3, Sb1
Break points/Insertion Site 98F5
Related Genes eIF4E-6 (CG1442), CG1951
Received Date 19 October 2007
Original Number LL00008
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: ~3R:24852220(+)
Cytological Band: 98F5
Gene Symbol-1: eIF4E-6
CG Number-1: CG1442
FlyBase ID-1: FBgn0039622
Insertion Type-1: 3' UTR
Gene Symbol-2: CG1951
CG Number-2: CG1951
FlyBase ID-2: FBgn0039623
Insertion Type-2: Putative Promoter.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]
Stock Request

Library & Clone Information
Strand Plus
Insertion Point 24852220
Chromosome Band 3R
Flanking Sequence
NNNNNNNNNNNGCATGCGNNNNTTTACGCNNACTATCTTTCNACGNGTAATAAGCTTTTA
AAAACGTTGAGGGATGTGCAATAGTTAGTTTATTCGTCTCTTTGGGATTTTAGCACTGTT
CGTTACATTAAATTCAGTGTACTTGTTTTGTTTATACTATTCAGTTGCCTGAACATAACA
TTGCAACATAAATTCAATTTGCGTAGAGCTCATGCGTAAAGAATTGCCAATTTTTATGCC
TTGTGAGGTTAACGACATCACAGAGCTTGTGACCAATTTCCCGGATGGCCTCATCGCCCG
CGTCTGCCTTTGTCCATACGGAGATTTTGTTGATATTTTTGCGAATCCTGACAAAAGCCC
CACAAATCTGATCGCAGTGATCGCAGGCCTCGCCAATCATGCAAAGAAGCACGTCCAGCC
AAGTTTTGTCCAGTTCGGCTGTGGACCTTTTATCCACTTTATATGTCCAACGACCTCCGC
CCTTGTTCGGCGGATCCTCCCACATGGGGCGAATGCCCTTCTTGAAGAGCATGTAATCAC
AGCCGCGGTTGAGCTTGGATGGCGTATCGAATTAACCATTGTGGGAACACTAGAACTAGC
TAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAATTCGCCCTATAGTGAGTCGTATT
ACGTTATCTAGTTAGGCGCGCCTGTGGGACGGAAGAACAGATGAATTAGATATCTATAAC
AAGAAATATATATATAATAAGTTATCACGTAAGTAGAACNTGAAATAACAATATAATTAT
CGTATGAGTTAAATCTTAAAAAGTCANGTAAAAGATAATCATGCGTCA
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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