Detailed Information [140007]
 

Strain Information
DGRC Number 140007
Genotype with FlyBase Link y[*] w[*]; PBac{SAstopDsRed}LL00022 P{ry[+t7.2]=neoFRT}40A P{w[+mW.hs]=FRT(w[hs])}G13 cn[1] bw[1] / CyO, S[*] bw[1]
Genotype y* w*; PBac{SAstopDsRed}LL00022 P{ry+t7.2=neoFRT}40A P{w+mW.hs=FRT(whs)}G13 cn1 bw1 / CyO, S* bw1
Break points/Insertion Site 28F5
Related Genes Spn2 (CG8137), CG14277
Received Date 19 October 2007
Original Number LL00022
Chromosome 2
Original Source Liqun Luo, Stanford University
Original Comments
Location: 2L:8241933(+)
Cytological Band: 28F5
Gene Symbol-1: Spn2
CG Number-1: CG8137
FlyBase ID-1: FBgn0028987
Insertion Type-1: 5' UTR
Gene Symbol-2: CG14277
CG Number-2: CG14277
FlyBase ID-2: FBgn0032008
Insertion Type-2: Putative Promoter.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) The combination of cn[1] bw[1] / CyO, S[*] bw[1] results in white eyes regardless of w[+] transgenes - therefore all flies should have white eyes when in stock - cross out to see red eye.
2) Third chromosome was never actively selected against, so y[+] flies may exist.
3) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]
Stock Request

Library & Clone Information
Strand Plus
Insertion Point 8241933
Chromosome Band 2L
Flanking Sequence 
NNNNNNNNANGCNTGCGNCATTTTACGCAGACTATCTTTCTAGGGTTAAATGTATAAATT
CCATAACAACATATGTAATTTGTCGAATTAACCATTGTGGGAACACTAGAACTAGCTAGT
TCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAATTCGCCCTATAGTGAGTCGTATTACGT
TATCTAGTTAGGCGCGCCTGTGGGACGGAAGAACAGATGAATTAGATATCTATAACAAGA
AAATATATATATAATAAGTTATCACGTAAGTAGAACATGAAATAACAATATAATTATCGT
ATGAGTTAAATCTTAAAAGTCACGTAAAAGATAATCATGCGTCA
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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