Detailed Information [140009]
 

Strain Information
DGRC Number 140009
Genotype with FlyBase Link y[*] w[*]; P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B PBac{SAstopDsRed}LL00040 P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM3, Sb[1]
Genotype y* w*; P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B PBac{SAstopDsRed}LL00040 P{y+t7.7 ry+t7.2=Car20y}96E / TM3, Sb1
Break points/Insertion Site 86C6
Related Genes Art1 (CG6554), Mcm5 (CG4082)
Received Date 19 October 2007
Original Number LL00040
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3R:6613922(+)
Cytological Band: 86C6
Gene Symbol-1: Art1
CG Number-1: CG6554
FlyBase ID-1: FBgn0037834
Insertion Type-1: 5' UTR
Gene Symbol-2: Mcm5
CG Number-2: CG4082
FlyBase ID-2: FBgn0017577
Insertion Type-2: Putative Promoter.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
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Library & Clone Information
Strand Plus
Insertion Point 6613922
Chromosome Band 3R
Flanking Sequence 
NNNNNNNNNGCATGCGTCATTTTACGCAGACTATCTTTCTAGGGTTAAAAGGTCGCTTAT
CAACACTGCCCCCGCACAAAAGCTGGAAACAAATATCGCCAGCTCGAATTAACCATTGTG
GGAACACTAGAACTAGCTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAATTCGCC
CTATAGTGAGTCGTATTACGTTATCTAGTTAGGCGCGCCTGTGGGACGGAAGAACAGATG
AATTAGATATCTATAACAAGAAAATATATATATAATAAGTTATCACGTAAGTAGAACATG
AAATAACAATATAATTATCGTATGAGTTAAATCTTAAAAGTCACGTAAAAGATAATCATG
CGTCA
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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