Detailed Information [140028]
 

Strain Information
DGRC Number 140028
Genotype with FlyBase Link y[*] w[*]; PBac{SAstopDsRed}trio[LL00125] P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM3, Sb[1]
Genotype y* w*; PBac{SAstopDsRed}trioLL00125 P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E / TM3, Sb1
Break points/Insertion Site 61e2
Related Genes trio (CG18214), CG9205
Received Date 19 October 2007
Original Number LL00125
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3L:1034856(+)
Cytological Band: 61e2
Gene Symbol-1: trio
CG Number-1: CG18214
FlyBase ID-1: FBgn0024277
Insertion Type-1: 5' UTR
Gene Symbol-2: CG9205
CG Number-2: CG9205
FlyBase ID-2: FBgn0035181
Insertion Type-2: 5' UTR.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Lethality lethal
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]
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Library & Clone Information
Strand Plus
Insertion Point 1034856
Chromosome Band 3L
Flanking Sequence 
GACTATCTTTCTAGGGTTAAACAAATTNGCAATTTTGCGAAACGTCAAGTGTGACCAGAT
GCCGGAACGGATTACAAACTGAATTTTCAGCCTTTTTTAATCGAATTAACCATTGTGGGA
ACACTAGAACTAGCTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAATTCGCCCTA
TAGTGAGTCGTATTACGTTATCTAGTTAGGCGCGCCTGTGGGACGGAAGAACAGATGAAT
TAGATATCTATAACAAGAAAATATATATATAATAAGTTATCACGTAAGTAGAACATGAAA
TAACAATATAATTATCGTATGAGTTAAATCTTAAAAGTCACGTAAAAGATAATCATGCGT
CA
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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