Detailed Information [140030]
 

Strain Information
DGRC Number 140030
Genotype with FlyBase Link y[*] w[*]; P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B PBac{SAstopDsRed}LL00145 P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM3, Sb[1]
Genotype y* w*; P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B PBac{SAstopDsRed}LL00145 P{y+t7.7 ry+t7.2=Car20y}96E / TM3, Sb1
Break points/Insertion Site 91A1
Related Genes l(3)05822 (CG7129)
Received Date 19 October 2007
Original Number LL00145
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3R:14121375(+)
Cytological Band: 91A1
Gene Symbol-1: l(3)05822
CG Number-1: CG7129
FlyBase ID-1: FBgn0010877
Insertion Type-1: Intron
Gene Symbol-2:
CG Number-2:
FlyBase ID-2:
Insertion Type-2:
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]
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Library & Clone Information
Strand Plus
Insertion Point 14121375
Chromosome Band 3R
Flanking Sequence 
NNNNNNNNNNGNANGCGTNATTTNTACGCNGACTATCTTTCTAGGNNNAAANNNNCTTCA
CTGAAACAAGAGAGAATGATCAAGTCGAATTAACCATTGTGGGAACACTAGAACTAGCTA
GTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAATTCGCCCTATAGTGAGTCGTATTAC
GTTATCTAGTTAGGCGCGCCTGTGGGACGGAAGAACAGATGAATTAGATATCTATAACAA
GAAAATATATATATAATAAGTTATCACGTAAGTAGAACATGAAATAACAATATAATTATC
GTATGAGTTAAATCTTAAAAGTCACGTAAAAGATAATCATGCGTCNNGATGATTTTGCAG
ATATTCGAATTAACCATTGTGGGAACACTAGAACTAGCTAGTTNTAGAGCGGCCGCCACC
GCGGTGGAGCTCCAATTCGCCCTATAGTGAGTCGTATTACGTTATCTAGTTAGGCGCGCC
TGTGGGACGGAAGAACAGATGAATTAGATATCTATAACAAGAAAATATATATATAATAAG
TTATCACGTAAGTAGAACATGAAATAACAATATAATTATCGTATGAGTTAAATCTTAAAA
GTCACGTAAAAGATAATCATGCGTCANNN
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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