Detailed Information [140048]
 

Strain Information
DGRC Number 140048
Genotype with FlyBase Link y[*] w[*]; PBac{SAstopDsRed}LL00227 P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM3, Sb[1]
Genotype y* w*; PBac{SAstopDsRed}LL00227 P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E / TM3, Sb1
Break points/Insertion Site 65F7
Related Genes SMSr (CG32380)
Received Date 19 October 2007
Original Number LL00227
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3L:7370080(-)
Cytological Band: 65F7
Gene Symbol-1: SMSr
CG Number-1: CG32380
FlyBase ID-1: FBgn0052380
Insertion Type-1: Intron
Gene Symbol-2:
CG Number-2:
FlyBase ID-2:
Insertion Type-2:
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
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Library & Clone Information
Strand Minus
Insertion Point 7370080
Chromosome Band 3L
Flanking Sequence 
NNNNANNNNNNGCATGCGTCATTTTACGCAGACTATCTTTCTAGGGTTAAATCCCATTTA
TAGTTTCTGTACTACTACCTATTCTACGAAAGTAAACGAATTTACCTTCGAATTAACCAT
TGTGGGAACACTAGAACTAGCTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAATT
CGCCCTATAGTGAGTCGTATTACGTTATCTAGTTAGGCGCGCCTGTGGGACGGAAGAACA
GATGAATTAGATATCTATAACAAGAAAATATATATATAATAAGTTATCACGTAAGTAGAA
CATGAAATAACAATATAATTATCGTATGAGTTAAATCTTAAAAGTCACGTAAAAGATAAT
CATGCGTCA
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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