Detailed Information [140049]
 

Strain Information
DGRC Number 140049
Genotype with FlyBase Link y[*] w[*]; PBac{SAstopDsRed}LL00228 P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; PBac{SAstopDsRed}LL00228 P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 70F6
Related Genes NHP2 (CG5258)
Received Date 19 October 2007
Original Number LL00228
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3L:14794975(+)
Cytological Band: 70F6
Gene Symbol-1: NHP2
CG Number-1: CG5258
FlyBase ID-1: FBgn0029148
Insertion Type-1: CDS
Gene Symbol-2:
CG Number-2:
FlyBase ID-2:
Insertion Type-2:
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Lethality lethal
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]
Stock Request

Library & Clone Information
Strand Plus
Insertion Point 14794975
Chromosome Band 3L
Flanking Sequence 
NNNNNNNNNANNNNNGCGTCATTTNACGCAGACTATCTTTCTAGGANNAAGTCCATTTCN
CAGGAAAGTCTTGTGCTTCATGGCCTTCTTCACCAGTTTGTAGCACTTTTTGGCCAGTTT
TTTACCTGCCATCGGTTTCGCGATGGCGTTCACAAAGATCAGCTTGTCGTCGTAGGACTC
CTCCTCCTTGATGGTAACATCGCCGCTGGCCACAACGGACTCATCGGCATCCTCGGAGCG
CTCTACTTTCACTTTGCCCATTGCTTTTTTGATAAAAACGTGGATATTTTGTTTAAACGG
CTTTTTGTTCGAATTAACCATTGTGGGAACACTAGAACTAGCTAGTTCTAGAGCGGCCGC
CACCGCGGTGGAGCTCCAATTCGCCCTATAGTGAGTCGTATTACGTTATCTAGTTAGGCG
CGCCTGTGGGACGGAAGAACAGATGAATTAGATATCTATAACAAGAAAATATATATATAA
TAAGTTATCACGTAAGTAGAACATGAAATANCNATATAATTATCCTATGAGTTAAATCTT
AAAAGTCACGTAAAAAATAATCATGCGTCA
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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