Detailed Information [140050]
 

Strain Information
DGRC Number 140050
Genotype with FlyBase Link y[*] w[*]; P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B PBac{SAstopDsRed}LL00229 P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM3, Sb[1]
Genotype y* w*; P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B PBac{SAstopDsRed}LL00229 P{y+t7.7 ry+t7.2=Car20y}96E / TM3, Sb1
Break points/Insertion Site 92F2
Related Genes Sep2 (CG4173), Srp72 (CG5434)
Received Date 19 October 2007
Original Number LL00229
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3R:16414165(-)
Cytological Band: 92F2
Gene Symbol-1: Sep2
CG Number-1: CG4173
FlyBase ID-1: FBgn0014029
Insertion Type-1: Putative Promoter
Gene Symbol-2: Srp72
CG Number-2: CG5434
FlyBase ID-2: FBgn0038810
Insertion Type-2: Putative Promoter.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]
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Library & Clone Information
Strand Minus
Insertion Point 16414165
Chromosome Band 3R
Flanking Sequence 
NNNNNNNNNNNNNNTGCNTCATTTNANNCANACTATCTTTCTAGGNNTAATTCTTTGTAA
AGTTATAATTTTCTTGTTCATCTATATCCACACACTTCTTTGAGCCGCTGGTATTTTGGG
CTCCGCGTATCTGGCCACACTTGCATATTCGCTGGCGACGCCTTTGATCCAAACTCTGCG
CCCGGATTATTGGATTATTTGACTTGACTATTGGAAAATGTCCGTTGAGGTGGATTTCGT
CGAATTAACCATTGTGGGAACACTAGAACTAGCTAGTTCTAGAGCGGCCGCCACCGCGGT
GGAGCTCCAATTCGCCCTATAGTGAGTCGTATTACGTTATCTAGTTAGGCGCGCCTGTGG
GACGGAAGAACAGATGAATTAGATATCTATAACAAGAAAATATATATATAATAAGTTATC
ACGTAAGTAGAACATGAAATAACAATATAATTATCGTATGAGTTAAATCTTAAAAGTCAC
GTAAAAGATAATCATGCGTCA
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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