Detailed Information [140053]
 

Strain Information
DGRC Number 140053
Genotype with FlyBase Link y[*] w[*]; P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E PBac{SAstopDsRed}LL00243 / TM3, Sb[1]
Genotype y* w*; P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E PBac{SAstopDsRed}LL00243 / TM3, Sb1
Break points/Insertion Site 96F3
Related Genes CG5886, CG14545
Received Date 19 October 2007
Original Number LL00243
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: ~3R:21677656(-)
Cytological Band: 96F3
Gene Symbol-1: CG5886
CG Number-1: CG5886
FlyBase ID-1: FBgn0039379
Insertion Type-1: CDS
Gene Symbol-2: CG14545
CG Number-2: CG14545
FlyBase ID-2: FBgn0040602
Insertion Type-2: Putative Promoter.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]
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Library & Clone Information
Strand Minus
Insertion Point 21677656
Chromosome Band 3R
Flanking Sequence 
NNNNNNNNNCATGCGTGAGTTAATCTTANAGTCACGTAAAAGATAATCATGCGTCATTTT
GACTCACGCGGTCGTTATAGTTCAAAATCAATGACACTTACCGCATTGACAATCACGCAT
GAGATAAATCTGAATCATCACGTAAAAGATAATCATGTTCTCATTTAAATTCACCCTTCC
CCTCGAATGAGGGTCATTGGATAAGGGATTTCTTGACCTAAAATAGATATAATTCCGCCT
TAAATTTTGTCTATTTATTCCCGTTTCCATGGATTCTGGCAGTCTATGGAAATAGGGTTT
GGTTCTGGCTTTTGGCCATCNCGCGCTCTTACCAACTCCTCCAACTTCTGGTCGCGCTGC
TTCTCCTCGCGCGCCACTTTTTTCGCCTTGCTTAGCTTCTCCATGGTCTATTCAAAAATC
GGTGCTATATTTAACTTGTATTCTATTCCTCTTTCGAATTAACCATTGTGGGAACACTAG
AACTANCTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCATTCGCCCTATAGTGAGT
CGTATTACGTTATCTAGNTAGGCGCGCCTGTGGGGACGGAAGAACAGATGAATTAGATAT
CTATAACCAAGAAAATATATATATAATAAGTTATCACGTAAGTAG
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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