Detailed Information [140055]
 

Strain Information
DGRC Number 140055
Genotype with FlyBase Link y[*] w[*]; P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B PBac{SAstopDsRed}LL00258 P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM3, Sb[1]
Genotype y* w*; P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B PBac{SAstopDsRed}LL00258 P{y+t7.7 ry+t7.2=Car20y}96E / TM3, Sb1
Break points/Insertion Site 85E9
Related Genes CG8516
Received Date 19 October 2007
Original Number LL00258
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: ~3R:5621273(-)
Cytological Band: 85E9
Gene Symbol-1: CG8516
CG Number-1: CG8516
FlyBase ID-1: FBgn0037757
Insertion Type-1: Intron
Gene Symbol-2:
CG Number-2:
FlyBase ID-2:
Insertion Type-2:
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Lethality semi lethal
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]
Stock Request

Library & Clone Information
Strand Minus
Insertion Point 5621273
Chromosome Band 3R
Flanking Sequence 
NNNNNNNNGCNNGCGTGATTTAACCCTNANTGTCNCGTCTAAGATNAACGGGCGTCANNA
GGTACTATGTGGTACTGGCAGACATTCTTACCTTAGCGTACATGCCCTCAATGTTTTTGT
TGCCCTCGCCTTCCTTGGAGGGACTTANACTTTTGCTATGCTTGCGCTGTGGCGACGCCT
CTCCAGATTCCGCCTCGGGTCCTTCCGCGCCCGCTTCGAATTAACCATTGTGGGAACACT
AGAACTAGCTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAATTCGCCCTATAGTG
AGTCGTATTACATTATCTAGTTAGGCGCGCCTGTGGGACGGAAGAACAGATGAATTATAT
ATCTATAACAAGAAAATATATATATAATAAGTTATCACGTAAGTAGAACATGAAATAACA
ATATAATTATCGTATGAGTTAAATCTTAAAAGTCACGTAAAAGATAATCATGCGTCA
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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