Detailed Information [140069]
 

Strain Information
DGRC Number 140069
Genotype with FlyBase Link y[*] w[*]; P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B PBac{SAstopDsRed}LL00311 P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM3, Sb[1]
Genotype y* w*; P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B PBac{SAstopDsRed}LL00311 P{y+t7.7 ry+t7.2=Car20y}96E / TM3, Sb1
Break points/Insertion Site 85D2
Related Genes CG9746
Received Date 19 October 2007
Original Number LL00311
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3R:5072214(+)
Cytological Band: 85D2
Gene Symbol-1: CG9746
CG Number-1: CG9746
FlyBase ID-1: FBgn0037663
Insertion Type-1: Putative Promoter
Gene Symbol-2:
CG Number-2:
FlyBase ID-2:
Insertion Type-2:
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]
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Library & Clone Information
Strand Plus
Insertion Point 5072214
Chromosome Band 3R
Flanking Sequence 
NNNNNNNNGCATGCGTCATTTNACGCANACTATCTTTCTAGGANNAACGAACTTGATATG
AGAACTTTAATTTAAGGTGCAAACCAATATATACCCTTGTATATAGCGGATATTCAATGT
TATTAAATATTTAATATAACTTTTATTTAGATTGAAAATGGGTATAGTGTGCATTGAGAA
TTCAATCGAATTAACCATTGTGGGAACACTAGAACTAGCTAGTTCTAGAGCGGCCGCCAC
CGCGGTGGAGCTCCAATTCGCCCTATAGTGAGTCGTATTACGTTATCTAGTTAGGCGCGC
CTGTGGGACGGAAGAACAGATGAATTAGATATCTATAACAAGAAAATATATATATAATAA
GTTATCACGTAAGTAGAACATGAAATAACAATATAATTATCGTATGAGTTAAATCTTAAA
AGTCACGTAAAAGATAATCATGCGTCATTTTGACTCACGCGGTCGTTATAGTTCAAAATC
AGTGACACTTACCGCATTGACAATCACGNTTTTNNNTAANTCTCNNC
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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