Detailed Information [140100]
 

Strain Information
DGRC Number 140100
Genotype with FlyBase Link y[*] w[*]; PBac{SAstopDsRed}LL00490 P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM3, Sb[1]
Genotype y* w*; PBac{SAstopDsRed}LL00490 P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E / TM3, Sb1
Break points/Insertion Site 66c5
Related Genes Rab-RP3 (CG7062)
Received Date 19 October 2007
Original Number LL00490
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3L:8291289(+)
Cytological Band: 66c5
Gene Symbol-1: Rab-RP3
CG Number-1: CG7062
FlyBase ID-1: FBgn0015793
Insertion Type-1: CDS
Gene Symbol-2:
CG Number-2:
FlyBase ID-2:
Insertion Type-2:
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
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Library & Clone Information
Strand Plus
Insertion Point 8291289
Chromosome Band 3L
Flanking Sequence 
NNNNNNNNNNNNNNNNNTCATTTTACGCNNACTATCTTTCTAGGGTTAAAGAGTTGCAGC
AGCTCATGCAATCTCACCTAGACCTAAATCCGCTCTTTAGTTTGCTTTTACTTTTGTGAT
CAAATTGTTCTTGTGGCTATATATTAGTATTCTAATGCTTTAACCTAATCCTAGACACTA
ACTTTTACACAACCATGTATTTATTTTTGACGATAGAGTACAAACAATCTCACACATCAG
CGAATCGAATTAACCATTGTGGGAACACTAGAACTAGCTAGTTCTAGAGCGGCCGCCACC
GCGGTGGAGCTCCAATTCGCCCTATAGTGAGTCGTATTACGTTATCTAGTTAGGCGCGCC
TGTGGGACGGAAGAACAGATGAATTAGATATCTATAACAAGAAAATATATATATAATAAG
TTATCACGTAAGTAGAACATGAAATAACAATATAATTATCGTATGAGTTAAATCTTAAAA
GTCACGTAAAAGATAATCATGCGTCAN
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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