Detailed Information [140111]
 

Strain Information
DGRC Number 140111
Genotype with FlyBase Link y[*] w[*]; PBac{SAstopDsRed}LL00581 P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM3, Sb[1]
Genotype y* w*; PBac{SAstopDsRed}LL00581 P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E / TM3, Sb1
Break points/Insertion Site 77b6
Related Genes Ide (CG5517), RhoBTB (CG5701)
Received Date 19 October 2007
Original Number LL00581
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3L:20368706(-)
Cytological Band: 77b6
Gene Symbol-1: Ide
CG Number-1: CG5517
FlyBase ID-1: FBgn0001247
Insertion Type-1: Intron
Gene Symbol-2: RhoBTB
CG Number-2: CG5701
FlyBase ID-2: FBgn0036980
Insertion Type-2: Putative Promoter.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2022-12-27
Research papers using this strain
[Please submit your publication]
Stock Request

Library & Clone Information
Strand Minus
Insertion Point 20368706
Chromosome Band 3L
Flanking Sequence 
NNNNNNNNNNNNGNNNNNANNNTTACGCNGACTATCTTTCTAGGGTTAAACAAATTTCCG
CTAACGGGCACCACTAAATCGCATTTATTTCGCACAGGTCAAGTGCGTTGAGGGCCAGCA
TTTCAGTAACACATCGAATTAACCATTGTGGGAACACTAGAACTAGCTAGTTCTAGAGCG
GCCGCCACCGCGGTGGAGCTCCAATTCGCCCTATAGTGAGTCGTATTACGTTATCTAGTT
AGGCGCGCCTGTGGGACGGAAGAACAGATGAATTAGATATCTATAACAAGAAAATATATA
TATAATAAGTTATCACGTAAGTAGAACATGAAATAACAATATAATTATCGTATGAGTTAA
ATCTTAAAAGTCACGTAAAAGATAATCATGCGTCA
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

CLOSE