Kyoto Drosophila Stock Center, Kyoto Institute of Technology

Strain Information
DGRC Number	140140
Genotype with FlyBase Link	y[] w[]; PBac{SAstopDsRed}LL00717, P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype	y^ w^; PBac{SAstopDsRed}LL00717, P{w^+mW.hs=FRT(w^hs)}2A P{ry^+t7.2=neoFRT}82B P{y^+t7.7 ry^+t7.2=Car20y}96E / TM6B, Tb¹
Break points/Insertion Site	96E2
Related Genes	CG5116, XNP (CG4548)
Received Date	19 October 2007
Original Number	LL00717
Chromosome	3
Comments	No Tb expression but Hu is OK. 21Jul2017 E.O.
Original Source	Liqun Luo, Stanford University
Original Comments	Location: 3R:21308998(-) Cytological Band: 96E2 Gene Symbol-1: CG5116 CG Number-1: CG5116 FlyBase ID-1: FBgn0039339 Insertion Type-1: Putative Promoter Gene Symbol-2: XNP CG Number-2: CG4548 FlyBase ID-2: FBgn0039338 Insertion Type-2: Putative Promoter. Location coordinates are based on the Drosophila Genome release 5.0. IMPORTANT NOTES: 1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population. 2) All third chromosome flies should be yellow[1] due to the insertion at 96E. 3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1]. 4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions. Oren Schuldiner (Weizmann Institute of Science)
General Information	MARCM
Genus	Drosophila
Subgenus	Sophophora
Species Group	melanogaster
Species Subgroup	melanogaster
Species	melanogaster
Reference	Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L. piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning. Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update	2020-04-03
Research papers using this strain [Please submit your publication]
Stock Request

Library & Clone Information

Strand	Minus
Insertion Point	21308998
Chromosome Band	3R
Flanking Sequence	tttacgcagactatctttctagggttaaACAAAATGTGGCGAGCCCCGTGAAAAGGGAGC GGGTCATGCAGTCCATGTAACCATcgaattaaccattgtgggaacactagaac
Sequence Comment	Location coordinates are based on the Drosophila Genome release 5.0.

ID
PWD