Detailed Information [140155]
 

Strain Information
DGRC Number 140155
Genotype with FlyBase Link y[*] w[*]; PBac{SAstopDsRed}LL00751 P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; PBac{SAstopDsRed}LL00751 P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 63B11
Related Genes CG32276, gry (CG17569)
Received Date 19 October 2007
Original Number LL00751
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3L:3198492(-)
Cytological Band: 63B11
Gene Symbol-1: CG32276
CG Number-1: CG32276
FlyBase ID-1: FBgn0047135
Insertion Type-1: CDS
Gene Symbol-2: gry
CG Number-2: CG17569
FlyBase ID-2: FBgn0035416
Insertion Type-2: Putative Promoter.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
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Library & Clone Information
Strand Minus
Insertion Point 3198492
Chromosome Band 3L
Flanking Sequence 
cgcagactatctttctagggttaaTGNAATATACAATATATTCATNNNNNNNNNNCAAGA
CTNNNNTGAACTTCTCTTTTTCGNNNNANNAANNGAGGGTCAATATCCGGTGGGTCCCTG
GCTCCTGGCGCTGTTCATCTTTGTGGTCTGCGGCTCTGCCATTTTCCAGATTGTTCAGTc
gaattaaccattgtgggaacactagaac
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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