Detailed Information [140217]
 

Strain Information
DGRC Number 140217
Genotype with FlyBase Link y[*] w[*]; P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B PBac{SAstopDsRed}LL00986 P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B PBac{SAstopDsRed}LL00986 P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 95F13
Related Genes CG5728, Dis3 (CG6413)
Received Date 19 October 2007
Original Number LL00986
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3R:20158371(+)
Cytological Band: 95F13
Gene Symbol-1: CG5728
CG Number-1: CG5728
FlyBase ID-1: FBgn0039182
Insertion Type-1: CDS
Gene Symbol-2: Dis3
CG Number-2: CG6413
FlyBase ID-2: FBgn0039183
Insertion Type-2: Putative Promoter.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Lethality lethal
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]
Stock Request

Library & Clone Information
Strand Plus
Insertion Point 20158371
Chromosome Band 3R
Flanking Sequence 
tttacgcagactatctttctagggttaaTGCTCTTGTGATGCAGATTACCGTGCACATCG
GCCGGCTTGAGGGAAAGGAGCAGGCTAACCCGACCGGTGTCCAGCTTTTCTGTTTTGATG
GCCTTGCCGTAGACGATGCGGCCCAACTGGAAGAGCTCGGTAAGGTCGTGGTACTCGCTG
GTGTCTCCGGACATGGCCGCCTTGGCCACACGGGTGTAGGCGTCGGATATATCCGCCACC
AAGGTGCGGGCGAACATCCGACCGGGCAAAGCGATCTGCAGTGCGGTGGCTGTCAACTCC
TTGACCACTCCCATGACCAGCATGTCCTCCTGCAGCGTGTCCATATTGAGCGTTTCCGCG
GAGAAGGCCTCCAGTTGGCCATTTTGTTCCTCGGTTTCGTAGCTCAGAAGGTTCTCTTTT
ACTTTCGGCGCCTTCTTCACCTTCTTCTGCGAGGCACCGAAAACCTGTGGGACAGAAAAG
TATGTACAAAGATCTCGGATATTTCGCTGGCTAAACTCACGATATTCAGGCTCACGTCGT
CGGTTTTGACTTCGCTGTGGATTGTCCCGCCTCGGGGAAAGCTTTTCTCGTTCGGCACCA
TCCGGGAGCTGTGTAATTTAGCGTTTTAAATAACAAAATTTGAGCAACGTGCGTTCTACA
CGTGCTAAGCGCCGATATAGAAAATCAGTTCAATAAATGGTACTAAATTATACCTTAAGA
TACGGGGCAGTCCGTAGACTCAAACCACTGTGACCATTGCGGCTGCAGTGTGAACAGGCC
TTAGGCAGATAATACCGCAGTcgaattaaccattgtgggaacactagaac
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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