Detailed Information [140261]
 

Strain Information
DGRC Number 140261
Genotype with FlyBase Link y[*] w[*]; PBac{SAstopDsRed}LL01116 P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; PBac{SAstopDsRed}LL01116 P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 65B4
Related Genes CG18769, CG18769
Received Date 19 October 2007
Original Number LL01116
Chromosome 3
Comments No Tb expression but Hu is OK. 2Nov2016 T.O.
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3L:6544494(+)
Cytological Band: 65B4
Gene Symbol-1: CG18769
CG Number-1: CG18769
FlyBase ID-1: FBgn0042185
Insertion Type-1: 5' UTR
Gene Symbol-2: CG18769
CG Number-2: CG18769
FlyBase ID-2: FBgn0042185
Insertion Type-2: Intron.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
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Stock Request

Library & Clone Information
Strand Plus
Insertion Point 6544494
Chromosome Band 3L
Flanking Sequence 
tttacgcagactatctttctagggttaaAGTAGTCAAAATTAAACGTAATCATGTYAAAA
TGTTAGGTGAAAGTGAAATTCGCATTGGCCGCTTTTKTTTATTTATCGTGGGAGCTATCT
ATTcgaattaaccattgtgggaacactagaac
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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