Detailed Information [140269]
 

Strain Information
DGRC Number 140269
Genotype with FlyBase Link y[*] w[*]; PBac{SAstopDsRed}LL01135 P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; PBac{SAstopDsRed}LL01135 P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 75E4
Related Genes CG3961, CG3961
Received Date 19 October 2007
Original Number LL01135
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3L:18881145(-)
Cytological Band: 75E4
Gene Symbol-1: CG3961
CG Number-1: CG3961
FlyBase ID-1: FBgn0036821
Insertion Type-1: Intron
Gene Symbol-2: CG3961
CG Number-2: CG3961
FlyBase ID-2: FBgn0036821
Insertion Type-2: Putative Promoter.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]
Stock Request

Library & Clone Information
Strand Minus
Insertion Point 18881145
Chromosome Band 3L
Flanking Sequence 
tttacgcagactatctttctagggttaaTTGCTGTCACTTGACTTGTCAGGGGCCCAGTG
GGAACWCCMGAACTAGCTAGTTCTAGAGCGGCCGCCACCGGGGTGGAGCTCCAATTCGCC
CTATGGGGAGTCGTATTGGGTTTTCTAGTTWGGCGCGCCGGTGGGACGGAAGAACAGATG
AATTATATATCTATMACAAGAAAATATATTTWTAATGGGTTATCACGCCCGTTTAACATG
GAATAGCAATATAATTATCGYATGAGTTAAATCTTAAAAGTCACGAAAAAKATAATGATG
CGTGATCTTCACTTGATTTTTTCTCAGGCTTTCTTCTGTAGGCAATCATTAAGCTAAACA
CGTAACATTCACTCAGGTTGAAAAGAAAAACAACTATTAACTACTACTGTAWTACCACAM
AAAAAAAAAAAYTMAATWTTTTAKGAAAAAAAKWARMCCCCCGAAAAAAMCGTTTKGGGC
CWACKMCAATCTTTWATTTGYWYCCAKTTTTTYTTTTTTYTKRGRTTWTTTKTWTAAAAT
TTTTWAAAATAKMGACWACCCGKAAGAGKTTTWAATAAAATATAACCTAWCYGRTWACWC
CAYWCAACAAAMWAAWTYCCYCAYWAGSTKGRTWAYTTAMTWTGTGATGAAAAGTARTTG
TTAYGAAMAWMAACCSAKGCSAKTTTATTAWAAAGWGTKAWCMATATTTGTTTATAWCAC
CCCACAAAAAAGTTKTSTTSMWAAAARTWGATGRTCTAWGTGTTTTAKAATASAWRAGAG
CCGCCAWAGAKASRKTTTTCKCCMCAAAAGATGGKTTSMGKAAAGGSKWGMTTKCAYCYT
CTATTGATGMCCCCCAYCRGGGGGGARRWTMSCWWRTAATTATTCTKTGAKGCAASWWAG
KAAYTCCCRTTCCMWTAATKKGGTCGMYTTWMMSWCSGWKYAAYCAGYSGMGCSCRCCKW
MMGGGCCARGRAAAYCSRATTAATMWMYTAWSYYYMGACRAAMTAGGGRSKGGMAYTTAA
WTMSMWA
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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