Detailed Information [140293]
 

Strain Information
DGRC Number 140293
Genotype with FlyBase Link y[*] w[*]; P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B PBac{SAstopDsRed}LL01235 P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B PBac{SAstopDsRed}LL01235 P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 83E5
Related Genes TfIIFalpha (CG10281), CG1024
Received Date 19 October 2007
Original Number LL01235
Chromosome 3
Comments No Tb expression but Hu is OK. 24May2017 E.O.
Original Source Liqun Luo, Stanford University
Original Comments
Location: ~3R:2206503(-)
Cytological Band: 83E5
Gene Symbol-1: TfIIFalpha
CG Number-1: CG10281
FlyBase ID-1: FBgn0010282
Insertion Type-1: CDS
Gene Symbol-2: CG1024
CG Number-2: CG1024
FlyBase ID-2: FBgn0027514
Insertion Type-2: Putative Promoter.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Lethality lethal
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]
Stock Request

Library & Clone Information
Strand Minus
Insertion Point 2206503
Chromosome Band 3R
Flanking Sequence 
CGTGNGTNAATCTTANAGTCACGTAAAANNNATCATGCGTCACGGGAGTTCCCGGCGGCG
ACTGAGATGTCCTAAATGCACAGCGACGGATTCGCGCTATTTAGAAAGAGAGAGCAATAT
TTCAGGAATGCATGCGTGAGTTAAATCTTAAAAGTCACGTAAAAGATAATCATGCGTCAC
GGGAGTTCCAAGCGGCGACGGAGATGTCCAAAATGCACAGCGGCGGATTCGATAATTTTG
CAAAAAGAGAGCAATATTTCCGGATTGCGTGCCTGGGTTACGCCTATCTCGGCCTCCTGC
AGGTTGGATGATGACNAGGCGGATCCTGAGGCGACAGAGGCTGCGGCGGCGGCAGCTGCG
GACGTGGAGCTCCCGCTGGCAGCGCTCGGCTGCATGAAATCAAGCGAAGACCGGAAATAA
GTGTTGGCGCCGGAAGCGGCAGGGTAAAGACTTACCGTcgaattaaccattgngggaaca
ctagaac
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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