Detailed Information [140387]
 

Strain Information
DGRC Number 140387
Genotype with FlyBase Link y[*] w[*]; PBac{SAstopDsRed}LL01510 P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; PBac{SAstopDsRed}LL01510 P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 69E2
Related Genes Atg1 (CG10967), CG17667
Received Date 19 October 2007
Original Number LL01510
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3L:12785119(-)
Cytological Band: "69E2"
Gene Symbol-1: Atg1
CG Number-1: CG10967
FlyBase ID-1: FBgn0010715
Insertion Type-1: Intron
Gene Symbol-2: CG17667
CG Number-2: CG17667
FlyBase ID-2: FBgn0036312
Insertion Type-2: Intron.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Lethality lethal
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]
Stock Request

Library & Clone Information
Strand Minus
Insertion Point 12785119
Chromosome Band 3L
Flanking Sequence 
tttacgcagactatctttctagggttaaGTAGAGTGTAAATTCGTGCACCTCTTCTWTAT
ATTAAAGGCGGCAATTAAGAGCGGTTGCCGTGGACYATYAATAAAATGTTGCTGTTTTTT
TTTTATAACCCCAKGSGKGRGTATYCCATTKGGMATKGATATCTCRGTKGKGGGSGGGTC
AARGYCCSGTTACCCKGGTTCACCKGTSGTTATTTSGTTTTTRGKGAATTAATCACKGSR
AAKGTKGCTAAASGRAAACTTKSCCCSGKGTCTYCCCKGTTTTTCGTTTTTRGRAARGGG
TCTMCKGATTTCWATAAATAAAKGGATWACTTATTTYCCRSCCCCATARGAATAACAAAK
GKRATTYCTCCACATATSGAATWAMCCATKGKGGRAMCAYTRRAAYTAGYTRKTTYTRRR
GSGGCCGCCMCSGCGGKGRRSCYCCAATYCGCCCTATRGKGRGTSGWATWACGTTAYYTR
GTTRGSSGCGCCKGTGGRACGRAARAMCAGATRAWTTARATWTCTATAACAGGAAAAWAW
TTWTATAAAAAGTTAYCACGWAAGWAGACMAKGAAAAAAMAGTAAAATTATCGTWTGAGT
WAAATCTAAAAGTCACGWKAAGGTCACTTATGSTTCCTGGAAAGYTATSGTGTTTGAAAC
CACCGCCGGCGCMCTGATAGAATAWSKSYYTTWTTKTWAASRMSMGSMSGSAWRMGRGYR
MWYRWTTYTTRRWWMASKKKSWWWWMA
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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