Detailed Information [140394]
 

Strain Information
DGRC Number 140394
Genotype with FlyBase Link y[*] w[*]; P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B PBac{SAstopDsRed}LL01524 P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B PBac{SAstopDsRed}LL01524 P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 84B2
Related Genes CG1965, CG1105
Received Date 19 October 2007
Original Number LL01524
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3R:2893940(-)
Cytological Band: "84B2"
Gene Symbol-1: CG1965
CG Number-1: CG1965
FlyBase ID-1: FBgn0037466
Insertion Type-1: Intron
Gene Symbol-2: CG1105
CG Number-2: CG1105
FlyBase ID-2: FBgn0037465
Insertion Type-2: Putative Promoter.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Lethality lethal
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
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Library & Clone Information
Strand Minus
Insertion Point 2893940
Chromosome Band 3R
Flanking Sequence 
tttacgcagactatctttctagggttaaTAACACATTTAAACTCATTCAGCCYTCRYWWK
GSKGCTAATTGTTCTACGATAATTCAATGTAATTTCTGWTGTTGAAATTTCGTTAAACGG
TATTATTGTAGAAGAAATTGAATAATAATAACCTTACCACAAAGTCGTCTGTGCGTATTT
CGGTTTGGATCATATGATTATCACTTTTTTTACTTTTACTCTGATCGCTGGCACTTTTAT
ATCTGCCAGCATTGGCAGGATTcgaattaaccattgtgggaacactagaac
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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