Detailed Information [140446]
 

Strain Information
DGRC Number 140446
Genotype with FlyBase Link y[*] w[*]; P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B PBac{SAstopDsRed}LL01687 P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B PBac{SAstopDsRed}LL01687 P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 87B11
Related Genes CG18549
Received Date 19 October 2007
Original Number LL01687
Chromosome 3
Comments No or little expression of Tb, but Hu is OK. M.T. 15May2013
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3R:8277244(-)
Cytological Band: "87B11"
Gene Symbol-1: CG18549
CG Number-1: CG18549
FlyBase ID-1: FBgn0038053
Insertion Type-1: Intron
Gene Symbol-2:
CG Number-2:
FlyBase ID-2:
Insertion Type-2:
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
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Library & Clone Information
Strand Minus
Insertion Point 8277244
Chromosome Band 3R
Flanking Sequence 
cagactatctttctagggttaaAATTATAATTAGGTCCAACTATTTGTACTGTGTAGCTA
TAAGTTTAGCTAACTACGCCAATGCACGCCCTGATTGGAATGTACTTTTGCTTTATTTTT
TTTTTTTTTTNNNGGGGCCGAATAAATTTTTTTAAAAAAAAAAAACCCACGGGGGGGGGG
GGGGGGCCCCAAAAAAAAAAAAAAANGTTCCCAAAAAAAAAAACCGGGNNAAACAAAAAA
TANNCTTGGGGTTAAAANTTCCNNNATTTNTTTTTTTTNGCNNCTTTTTTTTNNGGGGCC
CCCNANAAANNNNNNCNNNNNNNNNNGNNGTNNNAANCNAANAAANNCCNNAAAANNNGN
NNTTTNNAAAAAAAAAAANNNAAAAAANNNTTTNGGGNCNNTTNNGNNTNNAANTANCNN
NGGGGAANNNNNNANNNNNNNNTNNNNNGNNNCNCCCCNNNNGGNNCCCNNNNCCCCCNA
NNGNNNNNNNNNNNNNNNNNNNNNNNNNNCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNANNNNNNNNNNNNNNNTTNNCNNNNNNNAAANNNNANNNNNNNNNNNNNNN
NNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNA
NNANNNAA
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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