Detailed Information [140456]
 

Strain Information
DGRC Number 140456
Genotype with FlyBase Link y[*] w[*]; PBac{SAstopDsRed}LL01756 P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; PBac{SAstopDsRed}LL01756 P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 69D6
Related Genes snRNP69D (CG10753), Ptp69D (CG10975)
Received Date 19 October 2007
Original Number LL01756
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3L:12727897(-)
Cytological Band: "69D6"
Gene Symbol-1: snRNP69D
CG Number-1: CG10753
FlyBase ID-1: FBgn0016940
Insertion Type-1: Putative Promoter
Gene Symbol-2: Ptp69D
CG Number-2: CG10975
FlyBase ID-2: FBgn0014007
Insertion Type-2: Putative Promoter.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]
Stock Request

Library & Clone Information
Strand Minus
Insertion Point 12727897
Chromosome Band 3L
Flanking Sequence 
tttacgcagactatctttctagggttaaTAGGACACAAGTTTTACTTATGGAGCAGTATG
ACATGCACCACTTACCTGATTGTTGCAAATTTATAATTTATTTTTGGAATGCATTTATTC
ATTTATTATTTCATATACACTTTTATAACGATTCCGTTTGTAAGCATGCGTCGGTTACAC
CGCCAATGAGCGTTGCTATCTGAGAGGTGAATTAACCCCAGCGACAACTTTCTAATTAGA
TGGGTAATAGCCGGCATTGAAAGTGCTCGAACTCTTATTGTTTTCAACATCAAACATATT
AAAACAAGAAAATAATATGGTATGCCATGATTCATTAACTTATCGCATCGCTAAATTTGT
TAGCGATGCGTGCAGGAGAGGTTGAGGTAGTTATTGAGTTCTGAGATACTACACAGTTAC
TTGCATGAGAGAGCTATGCTTCTATTTGTTATATTTGTACACGAAATTTAATTTTATACG
AAGGTGGTTTTGATCTGTTATAATCACAGTTTTCCGTTATTTACTAGAATATGGTAACTA
TTCTGGCACTATAAGAGCATTTTGCGTAAGGAGGAACTGAGCAATCCATGCAAAAAATGG
GGGGCTACTAAAAATGAGCGACAAATAAAGTCGTACTAAGGAGGGAAAGCAAGGTAAATA
AAAGTATATTAATCAAAATGATGCTTCATTACAATTATTGGATCAAATTTCCATCACCCG
AAAGATTTTTGTTCTACCTAAAAGAGCCTGCTTCTATAAAAGCTGCATCTTTTGGACATG
GTATTCCTTAATTCATTTACCTAACTTCCCGTTGAGCCTTACAGATCCCTAAGAAGCTTA
TTACGAGACCTGACTTCGAATGAACGTTGGGGGAACACAAGACTGGCTGGTAAGGGGCGC
CCGCACCGCGGTGACCTCCATTTGGCTTAGGTGGGGTGTATTAGTATCCCGGTGGCGCCG
CATGAGAAGAAAAAACAGATAATTATTTTTTTATAAAAGATATATTTTTTTTGAAGTGTT
GCACGAAGAGGAAAAGAAAGAAATGTATTTATTATTGGGATTTATATATTAAAGTACGGA
TAAAAAAATAGGGGATGGGGAGACATAGTCGGTGAAGGGAGTCTCACGAGTAGAGC
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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