Detailed Information [140540]
 

Strain Information
DGRC Number 140540
Genotype with FlyBase Link y[*] w[*]; P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B PBac{SAstopDsRed}LL01993 P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B PBac{SAstopDsRed}LL01993 P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 83A4
Related Genes mRpL44 (CG2109), Snr1 (CG1064)
Received Date 25 October 2007
Original Number LL01993
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3R:1296302(-)
Cytological Band: 83A4
Gene Symbol-1: mRpL44
CG Number-1: CG2109
FlyBase ID-1: FBgn0037330
Insertion Type-1: CDS
Gene Symbol-2: Snr1
CG Number-2: CG1064
FlyBase ID-2: FBgn0011715
Insertion Type-2: Putative Promoter.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Lethality lethal
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]
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Library & Clone Information
Strand Minus
Insertion Point 1296302
Chromosome Band 3R
Flanking Sequence 
tttacgcagactatctttctagggttaaAGATGTCATTCTTACGCAGTGCTGTTGGTCTC
CTGGCTCGTGTCAAGCTCTTGGAGGGCTCACCGACTGGTGAGTAACCCGGATTCACCTAA
TGCCAGTCACGTAAAAGATAATCATGCATCACGTAAAAGATAATCATGCGTCACGTTAAA
GATCATCATGGCTCCACGTAAAAGATAACCAGGCGTCACGTAAAAGATAATCATGCGTCA
CGTAAAAGATAATCATGCGTCACGTAAAAGATAATCATGCGTCACGTAAAAGATAATCAT
GCGTCACGTAAAAGATGATCCTGCGTCACGTAAAAGATGATCATGCGTCACGTAAAAGAG
CATCACGCTTCACGTTAACGAGCGTCACGGTTCACGTAAACGAGGCTTAAGGTTAAGGTA
AATGAGGCTCCAGCTTCACGTAAACGAGGCGCAAGGTTAAAGTTAATCATGCTCCAGGTT
AAGGTGAATCATGCTTCAGGTAAACGATAATCAGGCTTCAGTTTAAAGATGATCAGGTTC
ACGTAAAACGACCTCATGCTTCAGGTAAAAGAGGTCAGCTTACTTAAAGAGATCACGTAA
AGTAAACAGCTCAGGTAAGTTAACAGATATGTTAAGAAACGTGTTCGGTAAGATAATCAC
CTCACGTAAGTACCCGGTCACGTTCAGAAGTCACGTAAGATACAGCATCACGTAAGTTAT
CGTGCTCACGTAAGATATCATGCGTACGTAAGATATCAGCGTCACTAAAGTCATTAGCGT
AGGTAAATTCTTAGCTACGTTAGGATCATG
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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