Detailed Information [140597]
 

Strain Information
DGRC Number 140597
Genotype with FlyBase Link y[*] w[*]; P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B PBac{SAstopDsRed}LL02249 P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B PBac{SAstopDsRed}LL02249 P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 87A2
Related Genes Hsp70Aa (CG31366), Hsp70Ab (CG18743)
Received Date 25 October 2007
Original Number LL02249
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: ~3R:7782994(-)
Cytological Band: 87A2
Gene Symbol-1: Hsp70Aa
CG Number-1: CG31366
FlyBase ID-1: FBgn0013275
Insertion Type-1: Putative Promoter
Gene Symbol-2: Hsp70Ab
CG Number-2: CG18743
FlyBase ID-2: FBgn0013276
Insertion Type-2: Putative Promoter.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Lethality lethal
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]
Stock Request

Library & Clone Information
Strand Minus
Insertion Point 7782994
Chromosome Band 3R
Flanking Sequence 
GGGGAATGCATGGGGGTCACGTTTACGTGATGTATGCTGTCCTGGGGGCAGACATTTACA
TATATACATATGTATGTACATATATACATATACATAAATTATGTATGAATTTATACATAT
GCTTTAGAAAGTTGAGACCGAAAATGTACACATTATAACCAGAACAGATTTTATTTACAT
TGCCACGCCCCTTTGAGTTCTAACCATCCCAAAAATCTGTAAAGCTCCCAGATTATCCAA
AAATTTAAATGGTTTAAATGTAAATACCGCGCTTACAAATGTACATGTACATGCATATGT
ATGTATGTATATGTATGTAGTTCAATATCCCTTCCTGCAATAAAAAGATTCAAAAATACA
TATATCTTAGTATTTAACTGTATTTAACTTTAGTTTTCATAAAAGCCACGGTTTTTGCAT
ATGCTTTTGCATGCATAAGTATTTAAATCTGTGTGATGTTCTAACGTTTATATATAGACT
CAAAAGTGTGCAAACTTGGAATTTGTTGTTTATTTTCTTGTATATTTAATATTTTTAATT
TTTTTTTGATTTATAAGTAAAATAAATATTGTTTAATTATATTGAATAAAAAAATTGCGT
TTAATTAAGCAAAGAAACCTTCATGTTTACCTTTAAAGCGAAAAATTCAACTTATTTCAC
AGTGTAAACAGTTGACAACAACAGTCTTGACAACCTGTACGTAATTTTAAATTAAAAACA
CTAACAATCATCTGCATGCAATTGTCTGTATTAATCTAATAAATAAATAGCTTTTTTAAG
TTAGTATGTAAATACATTTTAAAGAATATCTTGTCAAAGTTCCATAGGCCTTTCTGGCGG
ACAACATCCGCATAACAAACCCTTcgaattaaccattgtgggaacactagaac
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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