Detailed Information [140606]
 

Strain Information
DGRC Number 140606
Genotype with FlyBase Link y[*] w[*]; PBac{SAstopDsRed}LL02264 P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; PBac{SAstopDsRed}LL02264 P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 75C1
Related Genes W (CG5123)
Received Date 25 October 2007
Original Number LL02264
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3L:18178569(-)
Cytological Band: 75C1
Gene Symbol-1: W
CG Number-1: CG5123
FlyBase ID-1: FBgn0003997
Insertion Type-1: 5' UTR
Gene Symbol-2:
CG Number-2:
FlyBase ID-2:
Insertion Type-2:
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
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Library & Clone Information
Strand Minus
Insertion Point 18178569
Chromosome Band 3L
Flanking Sequence 
tttacgcagactatctttctagggttaaATCAGTTCTAAACACGAAAAGGGTTGATGAAA
AACGCAGAAGAATCCGAAAAACTAACTAACCGAGCAAAAACTTGACTTGAGTGTTGTTTG
ACAAATCAGGAAAGATAAAAAACAAATCATAAGAAAAAACTGCACGAAAAATGAAAAAGT
TTCTAATATTCAAAATCTTGCACAAGAAATACAAAATCAATTAAAGTGAACTCTAACCAA
AAGTTGTACACAAAATAAAAAGCAAAACAAAGCAGCGAAGAACAATCACAAGAAGAGCAA
AGTGCCAACAAAGTGCAGGAAGGAAGGAAGCGGATAAGGACAAAAAGGAAGCCAGCACAC
ACACACACACACCCACACAATGGCCGTGCCCTTTTATTTGCCCGAGGCGGCGCCGATGAC
GTAGCGTcgaattaaccattgtgggaacactagaac
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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