Detailed Information [140624]
 

Strain Information
DGRC Number 140624
Genotype with FlyBase Link y[*] w[*]; PBac{SAstopDsRed}LL02386 P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; PBac{SAstopDsRed}LL02386 P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 76D8
Related Genes CG7646, Nca (CG7641)
Received Date 25 October 2007
Original Number LL02386
Chromosome 3
Comments No or little Tb expression but Hu is OK. 12Mar2021 H.K.
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3L:19985014(+)
Cytological Band: 76D8
Gene Symbol-1: CG7646
CG Number-1: CG7646
FlyBase ID-1: FBgn0036926
Insertion Type-1: Putative Promoter
Gene Symbol-2: Nca
CG Number-2: CG7641
FlyBase ID-2: FBgn0013303
Insertion Type-2: Putative Promoter.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2021-03-12
Research papers using this strain
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Library & Clone Information
Strand Plus
Insertion Point 19985014
Chromosome Band 3L
Flanking Sequence 
tttacgcagactatctttctagggttaaGCCACCCCTTACTTTGTTGTATTGATTGTGTT
TTGGTCGCGGAaccattgtgggaagacta
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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