Detailed Information [140636]
 

Strain Information
DGRC Number 140636
Genotype with FlyBase Link y[*] w[*]; P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B PBac{SAstopDsRed}LL02571 P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B PBac{SAstopDsRed}LL02571 P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 85D1
Related Genes pum (CG9755)
Received Date 25 October 2007
Original Number LL02571
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3R:5060324(-)
Cytological Band: 85D1
Gene Symbol-1: pum
CG Number-1: CG9755
FlyBase ID-1: FBgn0003165
Insertion Type-1: 5' UTR
Gene Symbol-2:
CG Number-2:
FlyBase ID-2: FBgn0003165
Insertion Type-2:
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]
Stock Request

Library & Clone Information
Strand Minus
Insertion Point 5060324
Chromosome Band 3R
Flanking Sequence 
tttacgcagactatctttctagggttaaTAAACTACATTAATAAACGGAAATTCCACAAT
TCTCAACAAGAAATCTGTAAAAATTCAAGTTTTAACCAATTCTCATACGTAATTAAATAA
ATAAAGTTCAGTTTTGTTAAGTCCCAAAAACAACAGCTGTGCAACTGTGTAAAATATTAT
TCATAATTTTGTGTAAATTTATGTAAAAAAAAAAAAAAAAAAAACCAACCCCCAAACCCC
AACCAAAAAAAACAAATTTAATTTTTTTTAACAAAAATTGGGGGAAAAATTTCACCCAAA
CCGGGAAAATAAAATTTAAAATTATTTAGCAAAAAATGGGGTTTCCTTTCCCACAAAAAA
AAAAAAACCAAAAAAAAAATTTAAAACTGAACCCAAAATTAAAAAAAAAATTTTTGGAAC
CTTTTTAAAATTTTCCCAAGCAATTATTTTGCAAAACCCCCGGGGGGTTCCTTTTTCTGC
CAATTAAAAAAAAATTTAAATTGGACAGGCCCCCCAAATTTTCCCCCCCCAAATCTTGAA
AAAACCCCCAAAAATTGCCTCCTTTCCCCCCCCCAAAATGCCATAACCAattaaccattg
tggg
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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